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Home / Primary Antibodies / Polyclonal Antibodies

Anti-PDIA5 Antibody Picoband®

SKU : BHA21003257

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Mfr.No.	A09898, A09898-10ug, A09898-Biotin, A09898-Cy3, A09898-Dylight488, A09898-Dylight550, A09898-Dylight594, A09898-FITC, A09898-HRP, A09898-APC, A09898-PE, A09898-iFluor647, A09898-carrier-free
Alternative Names	interleukin 36, alpha
Background	This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alteative splicing results in multiple protein- and non-protein-coding transcript variants.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-PDIA5 Antibody Picoband® catalog # A09898. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in tu activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in
Host	Rabbit
Immunogen Region	E.coli-derived human PDIA5 recombinant protein (Position: R46-Y481).
Isotype	Rabbit IgG
Molecular Weight	60 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Interleukin-36 alpha; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6
Tissue Specificity	Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	Q14554
Gene Id	10954
Gene Name	PDIA5
Subcellular Location	Secreted.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Hartz, P. A. Personal Communication. Baltimore, Md. 5/9/2016.<br /><br />2. Hayano, T., Kikuchi, M. Molecular cloning of the cDNA encoding a novel protein disulfide isomerase-related protein (PDIR). FEBS Lett. 372: 210-214, 1995.<br /><br />3. Higa, A., Taouji, S., Lhomond, S., Jensen, D., Feandez-Zapico, M. E., Simpson, J. C., Pasquet, J.-M., Schekman, R., Chevet, E. Endoplasmic reticulum stress-activated transcription factor ATF6-alpha requires the disulfide isomerase PDIA5 to modulate chemoresistance. Molec. Cell. Biol. 34: 1839-1849, 2014.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003257-1	A09898	-	100 ug	$370		Add to Cart
BHA21003257-2	A09898-10ug	Sample	10 ug	$99		Add to Cart
BHA21003257-3	A09898-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003257-4	A09898-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003257-5	A09898-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003257-6	A09898-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003257-7	A09898-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003257-8	A09898-FITC	FITC	100 ug	$570		Add to Cart
BHA21003257-9	A09898-HRP	HRP	100 ug	$570		Add to Cart
BHA21003257-10	A09898-APC	APC	100 ug	$670		Add to Cart
BHA21003257-11	A09898-PE	PE	100 ug	$670		Add to Cart
BHA21003257-12	A09898-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003257-13	A09898-carrier-free	carrier-free	100 ug	$370		Add to Cart

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