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Home / Primary Antibodies / Polyclonal Antibodies

Anti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband®

SKU : BHA21000669

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Mfr.No.	PB9767, PB9767-10ug, PB9767-Biotin, PB9767-Cy3, PB9767-Dylight488, PB9767-Dylight550, PB9767-Dylight594, PB9767-FITC, PB9767-HRP, PB9767-APC, PB9767-PE, PB9767-iFluor647, PB9767-carrier-free
Alternative Names	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Background	O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) is an enzyme that in humans is encoded by the OGT gene. This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alteatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband® catalog # PB9767. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and divers
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human OGT, identical to the related mouse and rat sequences.
Isotype	Rabbit IgG
Molecular Weight	117 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit;2.4.1.255 ;O-GlcNAc transferase subunit p110;O-linked N-acetylglucosamine transferase 110 kDa subunit;OGT;OGT;
Tissue Specificity	Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	O15294
Gene Id	8473
Gene Name	OGT
Subcellular Location	Isoform 2: Mitochondrion. Membrane. Associates with the mitochondrial inner membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2ug/ml, Human Flow Cytometry (Fixed), 1-3ug/1x10⁶ cells, Human, Mouse

Reference	1. "Entrez Gene: OGT O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine: polypeptide-N -acetylglucosaminyl transferase)". 2. Lubas WA, Frank DW, Krause M, Hanover JA (Apr 1997). "O-Linked GlcNAc transferase is a conserved nucleocytoplasmic protein containing tetratricopeptide repeats". The Joual of Biological Chemistry 272(14): 931624. 3. Yang X, Ongusaha PP, Miles PD, Havstad JC, Zhang F, So WV, Kudlow JE, Michell RH, Olefsky JM, Field SJ, Evans RM (Feb 2008). "Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance". Nature 451 (7181): 9649.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000669-1	PB9767	100 ug	-	$370		Add to Cart
BHA21000669-2	PB9767-10ug	10 ug	Sample	$99		Add to Cart
BHA21000669-3	PB9767-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000669-4	PB9767-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000669-5	PB9767-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000669-6	PB9767-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000669-7	PB9767-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000669-8	PB9767-FITC	100 ug	FITC	$570		Add to Cart
BHA21000669-9	PB9767-HRP	100 ug	HRP	$570		Add to Cart
BHA21000669-10	PB9767-APC	100 ug	APC	$670		Add to Cart
BHA21000669-11	PB9767-PE	100 ug	PE	$670		Add to Cart
BHA21000669-12	PB9767-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000669-13	PB9767-carrier-free	100 ug	carrier-free	$370		Add to Cart

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