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Home / Primary Antibodies / Polyclonal Antibodies

Anti-NUP214 Antibody Picoband®

SKU : BHA21002426

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Mfr.No.	A02408, A02408-10ug, A02408-Biotin, A02408-Cy3, A02408-Dylight488, A02408-Dylight550, A02408-Dylight594, A02408-FITC, A02408-HRP, A02408-APC, A02408-PE, A02408-iFluor647, A02408-carrier-free
Alternative Names	nucleoporin 214
Background	Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t (6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alteative splicing of this gene results in multiple transcript variants encoding different isoforms.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-NUP214 Antibody Picoband® catalog # A02408. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
Host	Rabbit
Immunogen Region	E.coli-derived human NUP214 recombinant protein (Position: K36-E374).
Isotype	Rabbit IgG
Molecular Weight	280 kDa
Reactivity	Human, Mouse
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Nuclear pore complex protein Nup214; 214 kDa nucleoporin; Nucleoporin Nup214; Protein CAN; NUP214; CAIN, CAN, KIAA0023
Tissue Specificity	Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P35658
Gene Id	8021
Gene Name	NUP214
Subcellular Location	Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytosol . Cytoplasmic vesicle . Localizes at the cell membrane upon interaction with frizzled family members and promotes their intealization. Localizes to cytoplasmic puncta (By similarity). .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, ICC, WB
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human, Mouse<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human, Mouse<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Kraemer D, Wozniak RW, Blobel G, Radu A (Feb 1994). "The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm". Proceedings of the National Academy of Sciences of the United States of America. 91 (4): 151923.<br /><br />2. von Linde M, Poustka A, Lerach H, Grosveld G (Aug 1990). "The (6;9) chromosome translocation, associated with a specific subtype of acute nonlymphocytic leukemia, leads to aberrant transcription of a target gene on 9q34". Molecular and Cellular Biology. 10 (8): 401626.<br /><br />3. Napetschnig J, Blobel G, Hoelz A (2007). "Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN". Proc. Natl. Acad. Sci. U.S.A. 104 (6): 17838.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002426-1	A02408	-	100 ug	$370		Add to Cart
BHA21002426-2	A02408-10ug	Sample	10 ug	$99		Add to Cart
BHA21002426-3	A02408-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002426-4	A02408-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002426-5	A02408-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002426-6	A02408-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002426-7	A02408-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002426-8	A02408-FITC	FITC	100 ug	$570		Add to Cart
BHA21002426-9	A02408-HRP	HRP	100 ug	$570		Add to Cart
BHA21002426-10	A02408-APC	APC	100 ug	$670		Add to Cart
BHA21002426-11	A02408-PE	PE	100 ug	$670		Add to Cart
BHA21002426-12	A02408-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002426-13	A02408-carrier-free	carrier-free	100 ug	$370		Add to Cart

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