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Home / Primary Antibodies / Polyclonal Antibodies

Anti-NF-kB p65/RELA Antibody Picoband®

SKU : BHA21006670

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Mfr.No.	PA1125, PA1125-10ug, PA1125-Biotin, PA1125-Cy3, PA1125-Dylight488, PA1125-Dylight550, PA1125-Dylight594, PA1125-FITC, PA1125-HRP, PA1125-APC, PA1125-PE, PA1125-iFluor647, PA1125-carrier-free
Alternative Names	Transcription factor p65
Background	The p65 (RELA) heterodimer is the most abundant form of NFKB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A (PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B p65 through 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-NF-kB p65/RELA Antibody catalog # PA1125. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immuni
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human NF-kB p65, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	65 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;RELA;NFKB3;
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	Q04206
Gene Name	RELA
Sequence	Contains 1 RHD (Rel-like) domain.
Subcellular Location	Nucleus. Cytoplasm. Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity). Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation. .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br>

Reference	1. Deloukas, P.; van Loon, A. P. G. M. : Genomic organization of the gene encoding the p65 subunit of NF-kappa-B: multiple variants of the p65 protein may be generated by alteative splicing. Hum. Molec. Genet. 2: 1895-1900, 1993.<br /><br />2. Nickols, J. C.; Valentine, W.; Kanwal, S.; Carter, B. D. : Activation of the transcription factor NF-kappa-B in Schwann cells is required for peripheral myelin formation. Nature Neurosci. 6: 161-167, 2003. <br /><br />3. Zhong, H.; Voll, R. E.; Ghosh, S. : Phosphorylation of NF-kappa B by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Molec. Cell 1: 661-671, 1998.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006670-1	PA1125	-	100 ug	$370		Add to Cart
BHA21006670-2	PA1125-10ug	Sample	10 ug	$99		Add to Cart
BHA21006670-3	PA1125-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006670-4	PA1125-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006670-5	PA1125-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006670-6	PA1125-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006670-7	PA1125-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006670-8	PA1125-FITC	FITC	100 ug	$570		Add to Cart
BHA21006670-9	PA1125-HRP	HRP	100 ug	$570		Add to Cart
BHA21006670-10	PA1125-APC	APC	100 ug	$670		Add to Cart
BHA21006670-11	PA1125-PE	PE	100 ug	$670		Add to Cart
BHA21006670-12	PA1125-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006670-13	PA1125-carrier-free	carrier-free	100 ug	$370		Add to Cart

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