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Home / Primary Antibodies / Polyclonal Antibodies

Anti-NDUFA1 Antibody Picoband®

SKU : BHA21006250

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Mfr.No.	A08224, A08224-10ug, A08224-Biotin, A08224-Cy3, A08224-Dylight488, A08224-Dylight550, A08224-Dylight594, A08224-FITC, A08224-HRP, A08224-APC, A08224-PE, A08224-iFluor647, A08224-carrier-free
Background	NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Clonality	Polyclonal
Description	Boster Bio Anti-NDUFA1 Antibody Picoband® catalog # A08224. Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived human NDUFA1 recombinant protein (Position: M1-D70). Human NDUFA1 shares 82.9% amino acid (aa) sequence identity with mouse NDUFA1.
Molecular Weight	10 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	O15239
Gene Id	4694
Gene Name	NDUFA1

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.Avoid repeated freezing and thawing.

Applications	WB, IHC, ELISA
Description	Weste blot, 0.1-0.25 ug/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 ug/ml, Human ELISA, 0.1-0.5 ug/ml, -

Reference	1. Smeitink J, van den Heuvel L (1999). "Human mitochondrial complex I in health and disease.". Am. J. Hum. Genet. 64 (6): 150510. 2. Au, H. C., Seo, B. B., Matsuno-Yagi, A., Yagi, T., Scheffler, I. E. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria. Proc. Nat. Acad. Sci. 96: 4354-4359, 1999. 3. Feandez-Moreira, D., Ugalde, C., Smeets, R., Rodenburg, R. J. T., Lopez-Laso, E., Ruiz-Falco, M. L., Briones, P., Martin, M. A., Smeitink, J. A. M., Arenas, J. X-linked NDUFA1 gene mutations associated with mitochondrial encephalomyopathy. Ann. Neurol. 61: 73-83, 2007.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006250-1	A08224	-	100 ug	$370		Add to Cart
BHA21006250-2	A08224-10ug	Sample	10 ug	$99		Add to Cart
BHA21006250-3	A08224-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006250-4	A08224-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006250-5	A08224-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006250-6	A08224-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006250-7	A08224-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006250-8	A08224-FITC	FITC	100 ug	$570		Add to Cart
BHA21006250-9	A08224-HRP	HRP	100 ug	$570		Add to Cart
BHA21006250-10	A08224-APC	APC	100 ug	$670		Add to Cart
BHA21006250-11	A08224-PE	PE	100 ug	$670		Add to Cart
BHA21006250-12	A08224-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006250-13	A08224-carrier-free	carrier-free	100 ug	$370		Add to Cart

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