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Home / Primary Antibodies / Polyclonal Antibodies

Anti-MRPS25 Antibody Picoband®

SKU : BHA21003597

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Mfr.No.	A14080-3, A14080-3-10ug, A14080-3-Biotin, A14080-3-Cy3, A14080-3-Dylight488, A14080-3-Dylight550, A14080-3-Dylight594, A14080-3-FITC, A14080-3-HRP, A14080-3-APC, A14080-3-PE, A14080-3-iFluor647, A14080-3-carrier-free
Alternative Names	RNA-binding protein 47
Background	28S ribosomal protein S25, mitochondrial is a protein that in humans is encoded by the MRPS25 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alteative splicing results in multiple transcript variants.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-MRPS25 Antibody Picoband® catalog # A14080-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
Host	Rabbit
Immunogen Region	E.coli-derived human MRPS25 recombinant protein (Position: M1-D173).
Isotype	Rabbit IgG
Molecular Weight	20 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	RNA-binding protein 47;RNA-binding motif protein 47;RBM47;
Tissue Specificity	Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	P82663
Gene Id	64432
Gene Name	MRPS25
Subcellular Location	Nucleus .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.25 ug/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 ug/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells, Human ELISA, 0.1-0.5 ug/ml, -

Reference	1. Bugiardini, E., Mitchell, A. L., Dalla Rosa, I., Hoing-Do, H.-T., Pitmann, A. M., Poole, O. V., Holton, J. L., Shah, S., Woodward, C., Hargreaves, I., Quinlivan, R., Amunts, A., Wiesner, R. J., Houlden, H., Holt, I. J., Hanna, M. G., Pitceathly, R. D. S., Spinazzola, A. MRPS25 mutations impair mitochondrial translation and cause encephalomyopathy. Hum. Molec. Genet. 28: 2711-2719, 2019. 2. Kenmochi, N., Suzuki, T., Uechi, T., Magoori, M., Kuniba, M., Higa, S., Watanabe, K., Tanaka, T. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. Genomics 77: 65-70, 2001. 3. Koc, E. C., Burkhart, W., Blackbu, K., Moseley, A., Spremulli, L. L. The small subunit of the mammalian mitochondrial ribosome: identification of the full complement of ribosomal proteins present. J. Biol. Chem. 276: 19363-19374, 2001.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003597-1	A14080-3	-	100 ug	$370		Add to Cart
BHA21003597-2	A14080-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21003597-3	A14080-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003597-4	A14080-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003597-5	A14080-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003597-6	A14080-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003597-7	A14080-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003597-8	A14080-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21003597-9	A14080-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21003597-10	A14080-3-APC	APC	100 ug	$670		Add to Cart
BHA21003597-11	A14080-3-PE	PE	100 ug	$670		Add to Cart
BHA21003597-12	A14080-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003597-13	A14080-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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