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Home / Primary Antibodies / Polyclonal Antibodies

Anti-METTL3 Antibody Picoband®

SKU : BHA21003069

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Mfr.No.	A01758-2, A01758-2-10ug, A01758-2-Biotin, A01758-2-Cy3, A01758-2-Dylight488, A01758-2-Dylight550, A01758-2-Dylight594, A01758-2-FITC, A01758-2-HRP, A01758-2-APC, A01758-2-PE, A01758-2-iFluor647, A01758-2-carrier-free
Alternative Names	methyltransferase like 3
Background	N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by the METTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of inteal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-METTL3 Antibody Picoband® catalog # A01758-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	The METTL3-METTL14 heterodimer forms a N6- methyltransferase complex that methylates adenosine residues at the N (6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and haematopoietic stem ce
Host	Rabbit
Immunogen Region	E.coli-derived human METTL3 recombinant protein (Position: E71-L580).
Isotype	Rabbit IgG
Molecular Weight	64 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	N6-adenosine-methyltransferase catalytic subunit
Tissue Specificity	Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Uniprot	Q86U44
Gene Id	56339
Gene Name	METTL3
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Alarcon, C. R., Lee, H., Goodarzi, H., Halberg, N., Tavazoie, S. F. N(6)-methyladenosine marks primary microRNAs for processing. Nature 519: 482-485, 2015.<br /><br />2. Barbieri, I., Tzelepis, K., Pandolfini, L., Shi, J., Millan-Zambrano, G., Robson, S. C., Aspris, D., Migliori, V., Bannister, A. J., Han, N., De Braekeleer, E., Ponstingl, H., Hendrick, A., Vakoc, C. R., Vassiliou, G. S., Kouzarides, T.Promoter-bound METTL3 maintains myeloid leukaemia by m(6)A-dependent translation control. Nature 552: 126-131, 2017.<br /><br />3. Bujnicki, J. M., Feder, M., Radlinska, M., Blumenthal, R. M. Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA:m6A methyltransferase. J. Molec. Evol. 55: 431-444, 2002.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003069-1	A01758-2	-	100 ug	$370		Add to Cart
BHA21003069-2	A01758-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21003069-3	A01758-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003069-4	A01758-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003069-5	A01758-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003069-6	A01758-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003069-7	A01758-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003069-8	A01758-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21003069-9	A01758-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21003069-10	A01758-2-APC	APC	100 ug	$670		Add to Cart
BHA21003069-11	A01758-2-PE	PE	100 ug	$670		Add to Cart
BHA21003069-12	A01758-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003069-13	A01758-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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