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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband®

SKU : BHA21001047

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Mfr.No.	A00175, A00175-10ug, A00175-Biotin, A00175-Cy3, A00175-Dylight488, A00175-Dylight550, A00175-Dylight594, A00175-FITC, A00175-HRP, A00175-APC, A00175-PE, A00175-iFluor647, A00175-carrier-free
Alternative Names	Mannose-6-phosphate isomerase
Background	Mannose-6 phosphate isomerase (MPI), alteately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alteative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband® catalog # A00175. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Host	Rabbit
Immunogen Region	E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.
Isotype	Rabbit IgG
Molecular Weight	47 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Mannose-6-phosphate isomerase;5.3.1.8;Phosphohexomutase;Phosphomannose isomerase;PMI;MPI;PMI1;
Tissue Specificity	Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P34949
Gene Id	4351
Gene Name	MPI
Subcellular Location	Cytoplasm .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IHC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Jaeken, J., Matthijs, G., Saudubray, J.-M., Dionisi-Vici, C., Bertini, E., de Lonlay, P., Henri, H., Carchon, H., Schollen, E., Van Schaftingen, E. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation. (Letter) Am. J. Hum. Genet. 62: 1535-1539, 1998.<br /><br />2. Schollen, E., Dorland, L., de Koning, T. J., Van Diggelen, O. P., Huijmans, J. G. M., Marquardt, T., Babovic-Vuksanovic, D., Patterson, M., Imtiaz, F., Winchester, B., Adamowicz, M., Pronicka, E., Freeze, H., Matthijs, G. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib). Hum. Mutat. 16: 247-252, 2000.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001047-1	A00175	100 ug	-	$370		Add to Cart
BHA21001047-2	A00175-10ug	10 ug	Sample	$99		Add to Cart
BHA21001047-3	A00175-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001047-4	A00175-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001047-5	A00175-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001047-6	A00175-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001047-7	A00175-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001047-8	A00175-FITC	100 ug	FITC	$570		Add to Cart
BHA21001047-9	A00175-HRP	100 ug	HRP	$570		Add to Cart
BHA21001047-10	A00175-APC	100 ug	APC	$670		Add to Cart
BHA21001047-11	A00175-PE	100 ug	PE	$670		Add to Cart
BHA21001047-12	A00175-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001047-13	A00175-carrier-free	100 ug	carrier-free	$370		Add to Cart

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