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Home / Primary Antibodies / Polyclonal Antibodies

Anti-M-phase inducer phosphatase 2 Cdc25B Antibody Picoband®

SKU : BHA21006876

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Mfr.No.	PA1546, PA1546-10ug, PA1546-Biotin, PA1546-Cy3, PA1546-Dylight488, PA1546-Dylight550, PA1546-Dylight594, PA1546-FITC, PA1546-HRP, PA1546-APC, PA1546-PE, PA1546-iFluor647, PA1546-carrier-free
Alternative Names	M-phase inducer phosphatase 2
Background	Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-M-phase inducer phosphatase 2 Cdc25B Antibody catalog # PA1546. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. ly dephosphorylates CDK1 and stimulates
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25B, different from the related mouse sequence by three amino acids.
Isotype	Rabbit IgG
Molecular Weight	65 kDa
Reactivity	Rat, Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	M-phase inducer phosphatase 2;3.1.3.48;Dual specificity phosphatase Cdc25B;CDC25B;CDC25HU2;
Tissue Specificity	Isoform 2 is found in breast cancer tissues.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P30305
Gene Name	CDC25B
Sequence	Belongs to the MPI phosphatase family.
Subcellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, By Heat<br>Weste blot, 0.1-0.5ug/ml, Rat, Human<br>

Reference	1. Lane, S. A., Baker, E., Sutherland, G. R., Tonks, I., Hayward, N., Ellem, K.The human cell cycle gene CDC25B is located at 20p13.Genomics 15: 693-694, 1993.<br /><br />2. Bulavin, D. V., Higashimoto, Y., Popoff, I. J., Gaarde, W. A., Basrur, V., Potapova, O., Appella, E., Foace, A. J., Jr.Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase.Nature 411: 102-107, 2001.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006876-1	PA1546	-	100 ug	$370		Add to Cart
BHA21006876-2	PA1546-10ug	Sample	10 ug	$99		Add to Cart
BHA21006876-3	PA1546-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006876-4	PA1546-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006876-5	PA1546-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006876-6	PA1546-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006876-7	PA1546-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006876-8	PA1546-FITC	FITC	100 ug	$570		Add to Cart
BHA21006876-9	PA1546-HRP	HRP	100 ug	$570		Add to Cart
BHA21006876-10	PA1546-APC	APC	100 ug	$670		Add to Cart
BHA21006876-11	PA1546-PE	PE	100 ug	$670		Add to Cart
BHA21006876-12	PA1546-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006876-13	PA1546-carrier-free	carrier-free	100 ug	$370		Add to Cart

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