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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Lamin B1/LMNB1 Antibody Picoband®

SKU : BHA21000507

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Mfr.No.	PB9611, PB9611-10ug, PB9611-Biotin, PB9611-Cy3, PB9611-Dylight488, PB9611-Dylight550, PB9611-Dylight594, PB9611-FITC, PB9611-HRP, PB9611-APC, PB9611-PE, PB9611-iFluor647, PB9611-carrier-free
Alternative Names	Lamin-B1
Background	Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Lamin B1/LMNB1 Antibody Picoband® catalog # PB9611. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Host	Rabbit
Immunogen Region	E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.
Isotype	Rabbit IgG
Molecular Weight	67 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Lamin-B1;LMNB1;LMN2, LMNB;
Tissue Specificity	Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	P20700
Gene Id	4001
Gene Name	LMNB1
Sequence	Belongs to the intermediate filament family.
Subcellular Location	Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IP, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5 ug/ml Immunohistochemistry (Paraffin-embedded Section), 2-5 ug/ml, By Heat Immunocytochemistry/Immunofluorescence, 5 ug/ml Immunoprecipitation, 0.5-2 ug/ml Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells

Reference	1. Pollard, K. M.; Chan, E. K.; Grant, B. J.; Sullivan, K. E.; Tan, E. M.; Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990. 2. Tsai, M.-Y.; Wang, S.; Heidinger, J. M.; Shumaker, D. K.; Adam, S. A.; Goldman, R. D.; Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000507-1	PB9611	100 ug	-	$370		Add to Cart
BHA21000507-2	PB9611-10ug	10 ug	Sample	$99		Add to Cart
BHA21000507-3	PB9611-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000507-4	PB9611-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000507-5	PB9611-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000507-6	PB9611-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000507-7	PB9611-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000507-8	PB9611-FITC	100 ug	FITC	$570		Add to Cart
BHA21000507-9	PB9611-HRP	100 ug	HRP	$570		Add to Cart
BHA21000507-10	PB9611-APC	100 ug	APC	$670		Add to Cart
BHA21000507-11	PB9611-PE	100 ug	PE	$670		Add to Cart
BHA21000507-12	PB9611-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000507-13	PB9611-carrier-free	100 ug	carrier-free	$370		Add to Cart

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