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Home / Primary Antibodies / Polyclonal Antibodies

Anti-KAT3B/p300/EP300 Antibody Picoband®

SKU : BHA21003947

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Mfr.No.	A00117, A00117-10ug, A00117-Biotin, A00117-Cy3, A00117-Dylight488, A00117-Dylight550, A00117-Dylight594, A00117-FITC, A00117-HRP, A00117-APC, A00117-PE, A00117-iFluor647, A00117-carrier-free
Alternative Names	GTPase HRas
Background	E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-KAT3B/p300/EP300 Antibody Picoband® catalog # A00117. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. .
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence in the middle region of human KAT3B/p300/EP300, which shares 76.5% amino acid (aa) sequence identity with mouse EP300.
Isotype	Rabbit IgG
Molecular Weight	264-300 kDa
Reactivity	Human, Mouse
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;GTPase HRas, N-terminally processed;HRAS;HRAS1;
Tissue Specificity	Widely expressed. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q09472
Gene Id	2033
Gene Name	EP300
Subcellular Location	Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 ug/ml, Human Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells, Human

Reference	1. Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM (April 1994). "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor". Genes Dev. 8 (8): 86984. 2. Ogryzko VV et al. "The transcriptional coactivators p300 and CBP are histone acetyltransferases". Cell. 1996 87(5):953-9.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003947-1	A00117	-	100 ug	$370		Add to Cart
BHA21003947-2	A00117-10ug	Sample	10 ug	$99		Add to Cart
BHA21003947-3	A00117-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003947-4	A00117-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003947-5	A00117-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003947-6	A00117-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003947-7	A00117-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003947-8	A00117-FITC	FITC	100 ug	$570		Add to Cart
BHA21003947-9	A00117-HRP	HRP	100 ug	$570		Add to Cart
BHA21003947-10	A00117-APC	APC	100 ug	$670		Add to Cart
BHA21003947-11	A00117-PE	PE	100 ug	$670		Add to Cart
BHA21003947-12	A00117-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003947-13	A00117-carrier-free	carrier-free	100 ug	$370		Add to Cart

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