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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®

SKU : BHA21006682

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Mfr.No.	PA1214, PA1214-10ug, PA1214-Biotin, PA1214-Cy3, PA1214-Dylight488, PA1214-Dylight550, PA1214-Dylight594, PA1214-FITC, PA1214-HRP, PA1214-APC, PA1214-PE, PA1214-iFluor647, PA1214-carrier-free
Alternative Names	heat shock protein family A (Hsp70) member 1A/1B
Background	The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody catalog # PA1214. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and it
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence.
Isotype	Rabbit IgG
Molecular Weight	70 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1B
Tissue Specificity	Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P0DMV8/P0DMV9
Gene Name	HSPA8
Sequence	Belongs to the beta-catenin family.
Subcellular Location	Cytoplasm. Nucleus . Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, IHC-F, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Mouse, Rat<br> Immunocytochemistry , 0.5-1ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Tavaria M, Gabriele T, Kola I, Anderson RL (April 1996). "A hitchhiker's guide to the human Hsp70 family". Cell Stress Chaperones 1 (1): 238. <br /><br />2. Morano KA (October 2007). "New tricks for an old dog: the evolving world of Hsp70". Ann. N. Y. Acad. Sci. 1113: 114. <br /><br />3. Luders, J.; Demand, J.; Hohfeld, J. (2000), Joual of Biological Chemistry 275 (7): 4613461, http://www.jbc.org/cgi/content/full/275/7/4613, retrieved on 2009-04-07<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006682-1	PA1214	-	100 ug	$370		Add to Cart
BHA21006682-2	PA1214-10ug	Sample	10 ug	$99		Add to Cart
BHA21006682-3	PA1214-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006682-4	PA1214-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006682-5	PA1214-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006682-6	PA1214-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006682-7	PA1214-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006682-8	PA1214-FITC	FITC	100 ug	$570		Add to Cart
BHA21006682-9	PA1214-HRP	HRP	100 ug	$570		Add to Cart
BHA21006682-10	PA1214-APC	APC	100 ug	$670		Add to Cart
BHA21006682-11	PA1214-PE	PE	100 ug	$670		Add to Cart
BHA21006682-12	PA1214-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006682-13	PA1214-carrier-free	carrier-free	100 ug	$370		Add to Cart

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