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Home / Primary Antibodies / Polyclonal Antibodies

Anti-HIF-1-alpha/HIF1A Antibody Picoband®

SKU : BHA21000144

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Mfr.No.	PB9253, PB9253-10ug, PB9253-Biotin, PB9253-Cy3, PB9253-Dylight488, PB9253-Dylight550, PB9253-Dylight594, PB9253-FITC, PB9253-HRP, PB9253-APC, PB9253-PE, PB9253-iFluor647, PB9253-carrier-free
Alternative Names	Hypoxia-inducible factor 1-alpha
Background	HIF-1alpha (Hypoxia-inducible factor 1alpha, HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1alpha transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1alpha regulates the survival and function in the inflammatory microenvironment ly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-HIF-1-alpha/HIF1A Antibody Picoband® catalog # PB9253. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth fa
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminal of human HIF-1-alpha, different from the related mouse and rat sequences by three amino acids.
Isotype	Rabbit IgG
Molecular Weight	115-120 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS domain-containing protein 8;HIF1A;BHLHE78, MOP1, PASD8;
Tissue Specificity	Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q16665
Gene Id	3091
Gene Name	HIF1A
Sequence	Contains 1 bHLH (basic helix-loop-helix) domain.
Subcellular Location	Cytoplasm. Nucleus. Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat Weste blot, 0.1-0.5ug/ml, Human, Mouse

Reference	1. Sutter, C. H.; Laughner, E.; Semenza, G. L. : Hypoxia-inducible factor 1-alpha protein expression is controlled by oxygen-regulated ubiquitination that is disrupted by deletions and missense mutations. Proc. Nat. Acad. Sci. 97: 4748-4753, 2000. 2. Elson, D. A.; Thurston, G.; Huang, L. E.; Ginzinger, D. G.; McDonald, D. M.; Johnson, R. S.; Arbeit, J. M. : Induction of hypervascularity without leakage or inflammation in transgenic mice overexpressing hypoxia-inducible factor-1-alpha. Genes Dev. 15: 2520-2532, 2001. 3. Koshiji, M.; To, K. K.-W.; Hammer, S.; Kumamoto, K.; Harris, A. L.; Modrich, P.; Huang, L. E. : HIF-1-alpha induces genetic instability by transcriptionally downregulating MutS-alpha expression. Molec. Cell 17: 793-803, 2005. 4. Ivan, M.; Kondo, K.; Yang, H.; Kim, W.; Valiando, J.; Ohh, M.; Salic, A.; Asara, J. M.; Lane, W. S.; Kaelin, W. G., Jr. : HIF-alpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O(2) sensing. Science 292: 464-468, 2001.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000144-1	PB9253	100 ug	-	$370		Add to Cart
BHA21000144-2	PB9253-10ug	10 ug	Sample	$99		Add to Cart
BHA21000144-3	PB9253-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000144-4	PB9253-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000144-5	PB9253-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000144-6	PB9253-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000144-7	PB9253-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000144-8	PB9253-FITC	100 ug	FITC	$570		Add to Cart
BHA21000144-9	PB9253-HRP	100 ug	HRP	$570		Add to Cart
BHA21000144-10	PB9253-APC	100 ug	APC	$670		Add to Cart
BHA21000144-11	PB9253-PE	100 ug	PE	$670		Add to Cart
BHA21000144-12	PB9253-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000144-13	PB9253-carrier-free	100 ug	carrier-free	$370		Add to Cart

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