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Home / Primary Antibodies / Polyclonal Antibodies

Anti-HDJ2/DNAJA1 Antibody Picoband®

SKU : BHA21006916

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Mfr.No.	PA1597, PA1597-10ug, PA1597-Biotin, PA1597-Cy3, PA1597-Dylight488, PA1597-Dylight550, PA1597-Dylight594, PA1597-FITC, PA1597-HRP, PA1597-APC, PA1597-PE, PA1597-iFluor647, PA1597-carrier-free
Alternative Names	DnaJ homolog subfamily A member 1
Background	DNAJA1 (DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32% identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Weste blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-HDJ2/DNAJA1 Antibody catalog # PA1597. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co- chaperone. Functio
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2, identical to the related rat and mouse sequences.
Isotype	Rabbit IgG
Molecular Weight	45 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	DnaJ homolog subfamily A member 1;DnaJ protein homolog 2;HSDJ;Heat shock 40 kDa protein 4;Heat shock protein J2;HSJ-2;Human DnaJ protein 2;hDj-2;DNAJA1;DNAJ2, HDJ2, HSJ2, HSPF4;
Tissue Specificity	Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P31689
Gene Name	DNAJA1
Sequence	Contains 1 CR-type zinc finger.
Subcellular Location	Membrane ; Lipid-anchor . Cytoplasm . Microsome . Nucleus . Cytoplasm, perinuclear region . Mitochondrion . Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei. .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IF, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br>

Reference	1. Chellaiah, A., Davis, A., Mohanakumar, T. Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein. Biochim. Biophys. Acta 1174: 111-113, 1993.<br /><br />2. Banasavadi-Siddegowda YK et al. FKBP38 Peptidylprolyl Isomerase Promotes the Folding of Cystic Fibrosis Transmembrane Conductance Regulator in the Endoplasmic Reticulum. J Biol Chem 286:43071-80 (2011).<br /><br />3. Chellaiah A, Davis A, Mohanakumar T (Aug 1993). "Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein". Biochim Biophys Acta 1174 (1): 1113. <br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006916-1	PA1597	-	100 ug	$370		Add to Cart
BHA21006916-2	PA1597-10ug	Sample	10 ug	$99		Add to Cart
BHA21006916-3	PA1597-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006916-4	PA1597-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006916-5	PA1597-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006916-6	PA1597-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006916-7	PA1597-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006916-8	PA1597-FITC	FITC	100 ug	$570		Add to Cart
BHA21006916-9	PA1597-HRP	HRP	100 ug	$570		Add to Cart
BHA21006916-10	PA1597-APC	APC	100 ug	$670		Add to Cart
BHA21006916-11	PA1597-PE	PE	100 ug	$670		Add to Cart
BHA21006916-12	PA1597-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006916-13	PA1597-carrier-free	carrier-free	100 ug	$370		Add to Cart

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