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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GRP94/HSP90B1 Antibody Picoband®

SKU : BHA21000538

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Mfr.No.	PB9637, PB9637-10ug, PB9637-Biotin, PB9637-Cy3, PB9637-Dylight488, PB9637-Dylight550, PB9637-Dylight594, PB9637-FITC, PB9637-HRP, PB9637-APC, PB9637-PE, PB9637-iFluor647, PB9637-carrier-free
Alternative Names	Endoplasmin
Background	Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-GRP94/HSP90B1 Antibody Picoband® catalog # PB9637. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has
Host	Rabbit
Immunogen Region	E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.
Isotype	Rabbit IgG
Molecular Weight	100 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Endoplasmin;94 kDa glucose-regulated protein;GRP-94;Heat shock protein 90 kDa beta member 1;Tumor rejection antigen 1;gp96 homolog;HSP90B1;GRP94, TRA1;
Tissue Specificity	Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Uniprot	P14625
Gene Id	7184
Gene Name	HSP90B1
Sequence	Contains 1 HMG box DNA-binding domain.
Subcellular Location	Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2ug/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5ug/ml, Human Flow Cytometry (Fixed), 1-3ug/1x10⁶ cells, Human

Reference	1. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (December 2005). "The HSP90 family of genes in the human genome: insights into their divergence and evolution". Genomics 86 (6): 62737. 2. Maki RG, Old LJ, Srivastava PK (August 1990). "Human homologue of murine tumor rejection antigen gp96: 5'-regulatory and coding regions and relationship to stress-induced proteins". Proc. Natl. Acad. Sci. U.S.A.87 (15): 565862. 3. Randow F, Seed B (2001). "Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability.". Nat. Cell Biol. 3 (10): 8916.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000538-1	PB9637	100 ug	-	$370		Add to Cart
BHA21000538-2	PB9637-10ug	10 ug	Sample	$99		Add to Cart
BHA21000538-3	PB9637-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000538-4	PB9637-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000538-5	PB9637-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000538-6	PB9637-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000538-7	PB9637-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000538-8	PB9637-FITC	100 ug	FITC	$570		Add to Cart
BHA21000538-9	PB9637-HRP	100 ug	HRP	$570		Add to Cart
BHA21000538-10	PB9637-APC	100 ug	APC	$670		Add to Cart
BHA21000538-11	PB9637-PE	100 ug	PE	$670		Add to Cart
BHA21000538-12	PB9637-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000538-13	PB9637-carrier-free	100 ug	carrier-free	$370		Add to Cart

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