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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GILT/IFI30 Antibody Picoband®

SKU : BHA21002925

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Mfr.No.	A05754-2, A05754-2-10ug, A05754-2-Biotin, A05754-2-Cy3, A05754-2-Dylight488, A05754-2-Dylight550, A05754-2-Dylight594, A05754-2-FITC, A05754-2-HRP, A05754-2-APC, A05754-2-PE, A05754-2-iFluor647, A05754-2-carrier-free
Alternative Names	Protein phosphatase 1 regulatory subunit 14A
Background	Gamma-interferon-inducible lysosomal thiol reductase is an enzyme that, in humans, is encoded by the IFI30 gene. The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-GILT/IFI30 Antibody Picoband® catalog # A05754-2. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of mouse GILT/IFI30, which shares 84.2% and 89.5% amino acid (aa) sequence identity with human and rat GILT/IFI30, respectively.
Isotype	Rabbit IgG
Molecular Weight	28 kDa
Reactivity	Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1;Protein kinase C-potentiated inhibitor protein of 17 kDa;CPI-17;PPP1R14A;CPI17, PPP1INL;
Tissue Specificity	Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	Q9ESY9
Gene Id	65972
Gene Name	Ifi30
Subcellular Location	Cytoplasm .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IF, IHC, WB, Flow Cytometry
Description	Weste blot, 0.25-0.5ug/ml, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 1-2ug/ml, Mouse, Rat<br> Immunofluorescence, 5 ug/ml, Mouse, Rat<br> Flow Cytometry(Fixed), 1-3 ug/1x10<sup>6</sup> cells, Mouse<br>

Reference	1. Arunachalam, B., Phan, U. T., Geuze, H. J., Cresswell, P. Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT). Proc. Nat. Acad. Sci. 97: 745-750, 2000. <br /><br />2. Maric, M., Arunachalam, B., Phan, U. T., Dong, C., Garrett, W. S., Cannon, K. S., Alfonso, C., Karlsson, L., Flavell, R. A., Cressell, P. Defective antigen processing in GILT-free mice. Science 294: 1361-1365, 2001.<br /><br />3. Phan, U. T., Arunachalam, B., Cresswell, P. Gamma-interferon-inducible lysosomal thiol reductase (GILT): maturation, activity, and mechanism of action. J. Biol. Chem. 275: 25907-25914, 2000.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002925-1	A05754-2	-	100 ug	$370		Add to Cart
BHA21002925-2	A05754-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21002925-3	A05754-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002925-4	A05754-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002925-5	A05754-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002925-6	A05754-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002925-7	A05754-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002925-8	A05754-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21002925-9	A05754-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21002925-10	A05754-2-APC	APC	100 ug	$670		Add to Cart
BHA21002925-11	A05754-2-PE	PE	100 ug	$670		Add to Cart
BHA21002925-12	A05754-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002925-13	A05754-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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