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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GCLC Antibody Picoband®

SKU : BHA21006247

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Mfr.No.	A01722, A01722-10ug, A01722-Biotin, A01722-Cy3, A01722-Dylight488, A01722-Dylight550, A01722-Dylight594, A01722-FITC, A01722-HRP, A01722-APC, A01722-PE, A01722-iFluor647, A01722-carrier-free
Background	GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
Clonality	Polyclonal
Description	Boster Bio Anti-GCLC Antibody Picoband® catalog # A01722. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived human GCLC recombinant protein (Position: R24-H388). Human GCLC shares 94.2% and 93.7% amino acid (aa) sequence identity with mouse and rat GCLC, respectively.
Molecular Weight	73 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	P48506
Gene Id	2729
Gene Name	GCLC

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.Avoid repeated freezing and thawing.

Applications	ELISA, Flow Cytometry, IF, IHC, WB
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 ug/ml, Human Immunofluorescence, 5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells, Human ELISA, 0.1-0.5 ug/ml, -

Reference	1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006247-1	A01722	-	100 ug	$370		Add to Cart
BHA21006247-2	A01722-10ug	Sample	10 ug	$99		Add to Cart
BHA21006247-3	A01722-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006247-4	A01722-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006247-5	A01722-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006247-6	A01722-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006247-7	A01722-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006247-8	A01722-FITC	FITC	100 ug	$570		Add to Cart
BHA21006247-9	A01722-HRP	HRP	100 ug	$570		Add to Cart
BHA21006247-10	A01722-APC	APC	100 ug	$670		Add to Cart
BHA21006247-11	A01722-PE	PE	100 ug	$670		Add to Cart
BHA21006247-12	A01722-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006247-13	A01722-carrier-free	carrier-free	100 ug	$370		Add to Cart

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