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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GAD67/GAD1 Antibody Picoband®

SKU : BHA21000084

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Mfr.No.	PB9183, PB9183-10ug, PB9183-Biotin, PB9183-Cy3, PB9183-Dylight488, PB9183-Dylight550, PB9183-Dylight594, PB9183-FITC, PB9183-HRP, PB9183-APC, PB9183-PE, PB9183-iFluor647, PB9183-carrier-free
Alternative Names	Glutamate decarboxylase 1
Background	Glutamate decarboxylase 1 (brain, 67kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alteative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-GAD67/GAD1 Antibody Picoband® catalog # PB9183. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Catalyzes the production of GABA.
Host	Rabbit
Immunogen Region	E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.
Isotype	Rabbit IgG
Molecular Weight	67 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Glutamate decarboxylase 1;4.1.1.15;67 kDa glutamic acid decarboxylase;GAD-67;Glutamate decarboxylase 67 kDa isoform;GAD1;GAD, GAD67;
Tissue Specificity	Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q99259
Gene Id	2571
Gene Name	GAD1
Sequence	Belongs to the group II decarboxylase family.
Subcellular Location	Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat

Reference	1. Kelly, C. D.; Edwards, Y.; Johnstone, A. P.; Harfst, E.; Nogradi, A.; Nussey, S. S.; Povey, S.; Carter, N. D. : Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase. Ann. Hum. Genet. 56: 255-265, 1992. 2. Bu, D.-F.; Erlander, M. G.; Hitz, B. C.; Tillakaratne, N. J. K.; Kaufman, D. L.; Wagner-McPherson, C. B.; Evans, G. A.; Tobin, A. J. : Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. Proc. Nat. Acad. Sci. 89: 2115-2119, 1992. 3. "Entrez Gene: GAD1 glutamate decarboxylase 1 (brain, 67kDa)"

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000084-1	PB9183	100 ug	-	$370		Add to Cart
BHA21000084-2	PB9183-10ug	10 ug	Sample	$99		Add to Cart
BHA21000084-3	PB9183-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000084-4	PB9183-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000084-5	PB9183-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000084-6	PB9183-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000084-7	PB9183-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000084-8	PB9183-FITC	100 ug	FITC	$570		Add to Cart
BHA21000084-9	PB9183-HRP	100 ug	HRP	$570		Add to Cart
BHA21000084-10	PB9183-APC	100 ug	APC	$670		Add to Cart
BHA21000084-11	PB9183-PE	100 ug	PE	$670		Add to Cart
BHA21000084-12	PB9183-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000084-13	PB9183-carrier-free	100 ug	carrier-free	$370		Add to Cart

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