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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Flotillin 1/FLOT1 Antibody Picoband®

SKU : BHA21007252

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Mfr.No.	PA2033, PA2033-10ug, PA2033-Biotin, PA2033-Cy3, PA2033-Dylight488, PA2033-Dylight550, PA2033-Dylight594, PA2033-FITC, PA2033-HRP, PA2033-APC, PA2033-PE, PA2033-iFluor647, PA2033-carrier-free
Alternative Names	Flotillin-1
Background	FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The Inteational Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Flotillin 1/FLOT1 Antibody catalog # PA2033. Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1, different from the related rat and mouse sequences by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	47 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Flotillin-1;FLOT1;
Tissue Specificity	Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	O75955
Gene Name	FLOT1
Sequence	Belongs to the eIF-3 subunit B family.
Subcellular Location	Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IP, IHC, WB
Description	Weste blot, 0.1-0.5 ug/ml, Human, Mouse, Rat<br> Immunohistochemistry, 2-5 ug/ml, Human<br> Immunoprecipitation, 0.5-2 ug/ml, Human<br>

Reference	1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. <br /><br />2. Hazarika, P., Dham, N., Patel, P., Cho, M., Weidner, D., Goldsmith, L., Duvic, M. Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. J. Cell. Biochem. 75: 147-159, 1999.<br /><br />3. Santamaria, A., Castellanos, E., Gomez, V., Benedit, P., Renau-Piqueras, J., Morote, J., Reventos, J., Thomson, T. M., Paciucci, R. PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts. Molec. Cell. Biol. 25: 1900-1911, 2005.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21007252-1	PA2033	-	100 ug	$370		Add to Cart
BHA21007252-2	PA2033-10ug	Sample	10 ug	$99		Add to Cart
BHA21007252-3	PA2033-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21007252-4	PA2033-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21007252-5	PA2033-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21007252-6	PA2033-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21007252-7	PA2033-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21007252-8	PA2033-FITC	FITC	100 ug	$570		Add to Cart
BHA21007252-9	PA2033-HRP	HRP	100 ug	$570		Add to Cart
BHA21007252-10	PA2033-APC	APC	100 ug	$670		Add to Cart
BHA21007252-11	PA2033-PE	PE	100 ug	$670		Add to Cart
BHA21007252-12	PA2033-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21007252-13	PA2033-carrier-free	carrier-free	100 ug	$370		Add to Cart

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