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Home / Primary Antibodies / Polyclonal Antibodies

Anti-FH Antibody Picoband®

SKU : BHA21001305

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Mfr.No.	A02097, A02097-10ug, A02097-Biotin, A02097-Cy3, A02097-Dylight488, A02097-Dylight550, A02097-Dylight594, A02097-FITC, A02097-HRP, A02097-APC, A02097-PE, A02097-iFluor647, A02097-carrier-free
Alternative Names	fumarate hydratase
Background	Fumarase (or fumaratehydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle (TCA) or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. This enzyme participates in 2 metabolic pathways: citric acid cycle, reductive citric acid cycle (CO2 fixation), and is also important in renal cell carcinoma. Mutations in this gene have been associated with the development of leiomyomas in the skin and uterus in combination with renal cell carcinoma.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-FH Antibody Picoband® catalog # A02097. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Also acts as a tumor suppressor.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human FH, which shares 100% and 97.8% amino acid (aa) sequence identity with mouse and rat FH, respectively.
Isotype	Rabbit IgG
Molecular Weight	49 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Fumarate hydratase, mitochondrial; Fumarase; 4.2.1.2; FH;
Tissue Specificity	Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	FH: P07954
Gene Id	2271
Gene Name	FH
Subcellular Location	Isoform Mitochondrial: Mitochondrion.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, IHC-F, ICC, WB
Description	Western blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Immunohistochemistry (Frozen Section), 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 2ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells

Reference	1. Begley TP, McMurry J (2005). The organic chemistry of biological pathways. Roberts and Co. Publishers.<br /><br />2. Weaver T (October 2005). "Structure of free fumarase C from Escherichia coli". ActaCrystallogr. D. 61 (Pt 10): 1395401.<br /><br />

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001305-1	A02097	100 ug	-	$370		Add to Cart
BHA21001305-2	A02097-10ug	10 ug	Sample	$99		Add to Cart
BHA21001305-3	A02097-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001305-4	A02097-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001305-5	A02097-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001305-6	A02097-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001305-7	A02097-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001305-8	A02097-FITC	100 ug	FITC	$570		Add to Cart
BHA21001305-9	A02097-HRP	100 ug	HRP	$570		Add to Cart
BHA21001305-10	A02097-APC	100 ug	APC	$670		Add to Cart
BHA21001305-11	A02097-PE	100 ug	PE	$670		Add to Cart
BHA21001305-12	A02097-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001305-13	A02097-carrier-free	100 ug	carrier-free	$370		Add to Cart

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