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Home / Primary Antibodies / Polyclonal Antibodies

Anti-ERp57/PDIA3 Antibody Picoband®

SKU : BHA21006976

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Mfr.No.	PA1679, PA1679-10ug, PA1679-Biotin, PA1679-Cy3, PA1679-Dylight488, PA1679-Dylight550, PA1679-Dylight594, PA1679-FITC, PA1679-HRP, PA1679-APC, PA1679-PE, PA1679-iFluor647, PA1679-carrier-free
Alternative Names	Protein disulfide-isomerase A3
Background	PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-ERp57/PDIA3 Antibody catalog # PA1679. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence in the middle region of human ERp57, identical to the related rat and mouse sequences.
Isotype	Rabbit IgG
Molecular Weight	38 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Protein disulfide-isomerase A3;5.3.4.1;58 kDa glucose-regulated protein;58 kDa microsomal protein;p58;Disulfide isomerase ER-60;Endoplasmic reticulum resident protein 57;ER protein 57;ERp57;Endoplasmic reticulum resident protein 60;ER protein 60;ERp60;PDIA3;ERP57, ERP60, GRP58;
Tissue Specificity	Detected in the flagellum and head region of spermatozoa (at protein level). .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P30101
Gene Name	PDIA3
Sequence	Belongs to the protein disulfide isomerase family.
Subcellular Location	Endoplasmic reticulum. Endoplasmic reticulum lumen . Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat<br>Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br>

Reference	1. "Entrez Gene: PDIA3 protein disulfide isomerase family A, member 3".<br /><br />2. Ellerman, D. A., Myles, D. G., Primakoff, P. A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57. Dev. Cell 10: 831-837, 2006<br /><br />3. Garbi, N., Tanaka, S., Momburg, F., Hammerling, G. J. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nature Immun. 7: 93-102, 2006.<br /><br /><br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006976-1	PA1679	-	100 ug	$370		Add to Cart
BHA21006976-2	PA1679-10ug	Sample	10 ug	$99		Add to Cart
BHA21006976-3	PA1679-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006976-4	PA1679-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006976-5	PA1679-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006976-6	PA1679-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006976-7	PA1679-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006976-8	PA1679-FITC	FITC	100 ug	$570		Add to Cart
BHA21006976-9	PA1679-HRP	HRP	100 ug	$570		Add to Cart
BHA21006976-10	PA1679-APC	APC	100 ug	$670		Add to Cart
BHA21006976-11	PA1679-PE	PE	100 ug	$670		Add to Cart
BHA21006976-12	PA1679-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006976-13	PA1679-carrier-free	carrier-free	100 ug	$370		Add to Cart

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