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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Eph receptor A2/EPHA2 Antibody Picoband®

SKU : BHA21001498

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Mfr.No.	A00578, A00578-10ug, A00578-Biotin, A00578-Cy3, A00578-Dylight488, A00578-Dylight550, A00578-Dylight594, A00578-FITC, A00578-HRP, A00578-APC, A00578-PE, A00578-iFluor647, A00578-carrier-free
Alternative Names	EPH receptor A2
Background	EPHA2 (ephrin type-A receptor 2) also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By somatic cell hybrid analysis and fluorescence in situ hybridization, the EPHA2 gene is mapped to chromosome 1p36.1. EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1 (CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Eph receptor A2/EPHA2 Antibody Picoband® catalog # A00578. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent biional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as
Host	Rabbit
Immunogen Region	E. coli-derived human Eph receptor A2 recombinant protein (Position: M851-N970).
Isotype	Rabbit IgG
Molecular Weight	125 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA2; ECK
Tissue Specificity	Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	P29317
Gene Id	1969
Gene Name	EPHA2
Subcellular Location	Cell membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunocytochemistry/Immunofluorescence, 5ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br> ELISA, 0.1-0.5ug/ml<br>

Reference	1. Ganju, P., Shigemoto, K., Brennan, J., Entwistle, A., Reith, A. D. The Eck receptor tyrosine kinase is implicated in patte formation during gastrulation, hindbrain segmentation and limb development. Oncogene 9: 1613-1624, 1994.<br /><br />2. Jun, G., Guo, H., Klein, B. E. K., Klein, R., Wang, J. J., Mitchell, P., Miao, H., Lee, K. E., Joshi, T., Buck, M., Chugha, P., Bardenstein, D., and 9 others. EPHA2 is associated with age-related cortical cataract in mice and humans. PLoS Genet. 5: e1000584, 2009.<br /><br />3. Khounlotham, M., Subbian, S., Smith, R., III, Cirillo, S. L. G., Cirillo, J. D. Mycobacterium tuberculosis interferes with the response to infection by inducing the host EphA2 receptor. J. Infect. Dis. 199: 1797-1806, 2009.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001498-1	A00578	100 ug	-	$370		Add to Cart
BHA21001498-2	A00578-10ug	10 ug	Sample	$99		Add to Cart
BHA21001498-3	A00578-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001498-4	A00578-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001498-5	A00578-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001498-6	A00578-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001498-7	A00578-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001498-8	A00578-FITC	100 ug	FITC	$570		Add to Cart
BHA21001498-9	A00578-HRP	100 ug	HRP	$570		Add to Cart
BHA21001498-10	A00578-APC	100 ug	APC	$670		Add to Cart
BHA21001498-11	A00578-PE	100 ug	PE	$670		Add to Cart
BHA21001498-12	A00578-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001498-13	A00578-carrier-free	100 ug	carrier-free	$370		Add to Cart

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