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Home / Primary Antibodies / Polyclonal Antibodies

Anti-EIF3E Antibody Picoband®

SKU : BHA21003669

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Mfr.No.	A00481-4, A00481-4-10ug, A00481-4-Biotin, A00481-4-Cy3, A00481-4-Dylight488, A00481-4-Dylight550, A00481-4-Dylight594, A00481-4-FITC, A00481-4-HRP, A00481-4-APC, A00481-4-PE, A00481-4-iFluor647, A00481-4-carrier-free
Alternative Names	Eukaryotic translation initiation factor 3 subunit E
Background	Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-EIF3E Antibody Picoband® catalog # A00481-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, e
Host	Rabbit
Immunogen Region	E.coli-derived human EIF3E recombinant protein (Position: A160-M249).
Isotype	Rabbit IgG
Molecular Weight	52 kDa
Reactivity	Human, Mouse
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Eukaryotic translation initiation factor 3 subunit E ;eIF3e ;Eukaryotic translation initiation factor 3 subunit 6 ;Viral integration site protein INT-6 homolog;eIF-3 p48 ;EIF3E ;EIF3S6 , INT6 ;
Tissue Specificity	Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	P60228
Gene Id	3646
Gene Name	EIF3E
Subcellular Location	Cytoplasm. Nucleus, PML body.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB, IHC, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells, Human, Mouse ELISA, 0.1-0.5 ug/ml, -

Reference	1. Asano K, Merrick WC, Hershey JW (October 1997). "The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome". J. Biol. Chem. 272 (38): 2347780. 2. Miyazaki, S., Imatani, A., Ballard, L., Marchetti, A., Buttitta, F., Albertsen, H., Nevanlinna, H. A., Gallahan, D., Callahan, R. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. Genomics 46: 155-158, 1997.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003669-1	A00481-4	-	100 ug	$370		Add to Cart
BHA21003669-2	A00481-4-10ug	Sample	10 ug	$99		Add to Cart
BHA21003669-3	A00481-4-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003669-4	A00481-4-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003669-5	A00481-4-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003669-6	A00481-4-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003669-7	A00481-4-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003669-8	A00481-4-FITC	FITC	100 ug	$570		Add to Cart
BHA21003669-9	A00481-4-HRP	HRP	100 ug	$570		Add to Cart
BHA21003669-10	A00481-4-APC	APC	100 ug	$670		Add to Cart
BHA21003669-11	A00481-4-PE	PE	100 ug	$670		Add to Cart
BHA21003669-12	A00481-4-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003669-13	A00481-4-carrier-free	carrier-free	100 ug	$370		Add to Cart

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