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Home / Primary Antibodies / Polyclonal Antibodies

Anti-EGFR Antibody Picoband®

SKU : BHA21001235

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Mfr.No.	A00023, A00023-10ug, A00023-Biotin, A00023-Cy3, A00023-Dylight488, A00023-Dylight550, A00023-Dylight594, A00023-FITC, A00023-HRP, A00023-APC, A00023-PE, A00023-iFluor647, A00023-carrier-free
Alternative Names	Epidermal growth factor receptor
Background	The breakpoint cluster region protein (BCR) is a protein that in humans is encoded by the BCR gene. A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-EGFR Antibody Picoband® catalog # A00023. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human EGFR, different from the related mouse sequence by two amino acids.
Isotype	Rabbit IgG
Molecular Weight	175 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Epidermal growth factor receptor;2.7.10.1;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;EGFR;ERBB, ERBB1, HER1;
Tissue Specificity	Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P00533
Gene Id	1956
Gene Name	EGFR
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IHC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Herbst RS (2004). "Review of epidermal growth factor receptor biology". Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 216.<br /><br />2. Kuan CT, Wikstrand CJ, Bigner DD (June 2001). "EGF mutant receptor vIII as a molecular target in cancer therapy". Endocr. Relat. Cancer 8 (2): 8396.<br /><br />3. Wang, K.; Yamamoto, H.; Chin, J. R.; Werb, Z.; Vu, T. H.: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004.<br /><br />

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001235-1	A00023	100 ug	-	$370		Add to Cart
BHA21001235-2	A00023-10ug	10 ug	Sample	$99		Add to Cart
BHA21001235-3	A00023-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001235-4	A00023-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001235-5	A00023-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001235-6	A00023-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001235-7	A00023-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001235-8	A00023-FITC	100 ug	FITC	$570		Add to Cart
BHA21001235-9	A00023-HRP	100 ug	HRP	$570		Add to Cart
BHA21001235-10	A00023-APC	100 ug	APC	$670		Add to Cart
BHA21001235-11	A00023-PE	100 ug	PE	$670		Add to Cart
BHA21001235-12	A00023-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001235-13	A00023-carrier-free	100 ug	carrier-free	$370		Add to Cart

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