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Home / Primary Antibodies / Polyclonal Antibodies

Anti-EAAT3 (EAAC1) Antibody

SKU : BHA21300408

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Alomone Labs

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Mfr.No.	AGC-023, AGC-023-CF_0.2 ml, AGC-023_0.2 ml, AGC-023_50 mcl
Lead Time	1-2 Business Days
Immunogen Species	Rat
Background	L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular synaptic milieu leads to neuronal cell death by a process known as excitotoxicity.The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for the re-uptake of Glu into the cells1,2.The EAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of Na+-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2.The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functional unit capable of binding the Glu substrate.The transport of Glu into the cells by the EAAT transporters is coupled to the Na+ and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na+ and one H+ ions, and the counter transport of one K+ ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl- conductance. The physiological significance of the Cl- current through the EAATs is currently unknown1,2.EAAT2 as well as EAAT1 are expressed predominantly in glia cells, while EAAT3 (Excitatory Amino Acid Transporter 3), EAAT4 and EAAT5 are mostly expressed in neurons.As mentioned, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintenance of low and non-toxic concentrations of glutamate and the preservation of normal excitatory synaptic transmission.In addition to Glu uptake, the glutamate transporters provide glutamate for the synthesis of g-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism1,2.Dysregulation of EAAT activities has been implicated in several neurodegenerative disorders such as Alzheimer's disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions1,2.
Clonality	Polyclonal
Description	Anti-EAAT3 (EAAC1) Antibody (#AGC-023) is a highly specific antibody directed against an epitope of the rat Excitatory amino acid transporter 3. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize EAAT3 from rat, mouse, and human samples.
Formulation	PBS pH7.4
Host	Rabbit
Immunogen Region	Intracellular, C-terminus
Isotype	Rabbit IgG
Reactivity	Human, Rat, Mouse
Reconstitution	0.2 ml double distilled water (DDW).
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Synonyms	Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Sodium-dependent glutamate/aspartate transporter 3, SLC1A1
UNSPSC Code	41116161
Note	Homology: Mouse, human - 13/14 amino acid residues identical Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry Negative Control: BLP-GC023 Specifictiy: SLC1A1 Is Toxin: No Standard quality control of each lot: Western blot analysis Antigen preadsorption control: 1 ug peptide per 1 ug antibody

Accession Number	P51907
Gene Id	25550
Target	Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, Sodium-dependent glutamate/aspartate transporter 3, SLC1A1
Sequence	(C)EPTILDNEDSDTKK, corresponding to amino acid residues 487-500 of rat EAAT3

Conjugates	Unconjugated

Concentration	1 mg/ml
Form	Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Preservative	No Preservative
Purity	Affinity purified on immobilized antigen.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Applications	IC, IF, IHC, WB
Dilution	Immunohistochemistry paraffin embedded sections ihc: Contact Biohippo for information Western blot wb: 1:400

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA21300408-1	AGC-023-CF_0.2 ml	0.2 ml(Carrier Free)	$994		Add to Cart
BHA21300408-2	AGC-023_0.2 ml	0.2 ml	$795		Add to Cart
BHA21300408-3	AGC-023_50 mcl	50 mcl	$697		Add to Cart

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