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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Diubiquitin/UBD Antibody Picoband®

SKU : BHA21007407

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Mfr.No.	PA2222, PA2222-10ug, PA2222-Biotin, PA2222-Cy3, PA2222-Dylight488, PA2222-Dylight550, PA2222-Dylight594, PA2222-FITC, PA2222-HRP, PA2222-APC, PA2222-PE, PA2222-iFluor647, PA2222-carrier-free
Alternative Names	Ubiquitin D
Background	Ubiquitin D, also called UBD or FAT10 is a protein that in humans is encoded by the UBD gene. This gene is mapped to 6p22.1. Ubiquitin-like protein modifier can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome in a NUB1L-dependent manner. This gene may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. It may be involved in the formation of aggresomes when proteasome is saturated or impaired. This gene mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It probably functions as a survival factor.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Diubiquitin/UBD Antibody catalog # PA2222. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UB
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human Diubiquitin, different from the related mouse and rat sequences by five amino acids.
Isotype	Rabbit IgG
Molecular Weight	91 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Ubiquitin D;Diubiquitin;Ubiquitin-like protein FAT10;UBD;FAT10;
Tissue Specificity	Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	O15205
Gene Name	UBD
Sequence	Contains 2 ubiquitin-like domains.
Subcellular Location	Nucleus . Cytoplasm . Accumulates in aggresomes under proteasome inhibition conditions.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat<br>Weste blot, 0.1-0.5ug/ml, Human<br>

Reference	1. Bates, E. E. M., Ravel, O., Dieu, M.-C., Ho, S., Guret, C., Bridon, J.-M., Ait-Yahia, S., Briere, F., Caux, C., Banchereau, J., Lebecque, S. Identification and analysis of a novel member of the ubiquitin family expressed in dendritic cells and mature B cells. Europ. J. Immun. 27: 2471-2477, 1997.<br /><br />2. Buchsbaum, S., Bercovich, B., Ciechanover, A. FAT10 is a proteasomal degradation signal that is itself regulated by ubiquitination. Molec. Biol. Cell 23: 225-232, 2012.<br /><br />3. Chiu, Y.-H., Sun, Q., Chen, Z. J. E1-L2 activates both ubiquitin and FAT10. Molec. Cell 27: 1014-1023, 2007. <br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21007407-1	PA2222	-	100 ug	$370		Add to Cart
BHA21007407-2	PA2222-10ug	Sample	10 ug	$99		Add to Cart
BHA21007407-3	PA2222-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21007407-4	PA2222-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21007407-5	PA2222-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21007407-6	PA2222-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21007407-7	PA2222-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21007407-8	PA2222-FITC	FITC	100 ug	$570		Add to Cart
BHA21007407-9	PA2222-HRP	HRP	100 ug	$570		Add to Cart
BHA21007407-10	PA2222-APC	APC	100 ug	$670		Add to Cart
BHA21007407-11	PA2222-PE	PE	100 ug	$670		Add to Cart
BHA21007407-12	PA2222-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21007407-13	PA2222-carrier-free	carrier-free	100 ug	$370		Add to Cart

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