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Home / Primary Antibodies / Polyclonal Antibodies

Anti-DDX5 Antibody Picoband®

SKU : BHA21007199

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Mfr.No.	PA1964, PA1964-10ug, PA1964-Biotin, PA1964-Cy3, PA1964-Dylight488, PA1964-Dylight550, PA1964-Dylight594, PA1964-FITC, PA1964-HRP, PA1964-APC, PA1964-PE, PA1964-iFluor647, PA1964-carrier-free
Alternative Names	Probable ATP-dependent RNA helicase DDX5
Background	DDX5 (DEAD/H BOX 5), also known as HLR1 or G17P1, is an enzyme that in humans is encoded by the DDX5 gene. The p68 protein is a proliferation-associated nuclear antigen first identified through its highly specific cross-reaction with the simian virus 40 tumor antigen (Iggo et al., 1989). Subsequently, homology to eukaryotic translation initiation factor was found, and amino acid sequence blocks characteristic of a large superfamily of proteins with putative helicase activity were demonstrated. Brody et al. (1995) confirmed that this gene is located on chromosome 17 in the region of the BRCA1 gene at 17q21. By immunoprecipitation analysis, Caretti et al. (2006) found that p68, p72 (DDX17), and the noncoding RNA SRA (SRA1) associated with MYOD (MYOD1) in MYOD-transfected HeLa cells.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-DDX5 Antibody catalog # PA1964. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Involved in the alteative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The ra
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5, identical to the related rat and mouse sequences.
Isotype	Rabbit IgG
Molecular Weight	69 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Probable ATP-dependent RNA helicase DDX5;3.6.4.13;DEAD box protein 5;RNA helicase p68;DDX5;G17P1, HELR, HLR1;
Tissue Specificity	Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P17844
Gene Name	DDX5
Sequence	Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Subcellular Location	Nucleus, nucleolus .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, IHC-F, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human

Reference	1. Brody, L. C., Abel, K. J., Castilla, L. H., Couch, F. J., McKinley, D. R., Yin, G.-Y., Ho, P. P., Merajver, S., Chandrasekharappa, S. C., Xu, J., Cole, J. L., Struewing, J. P., Valdes, J. M., Collins, F. S., Weber, B. L. Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics 25: 238-247, 1995.<br /><br />2. Caretti, G., Schiltz, R. L., Dilworth, F. J., Di Padova, M., Zhao, P., Ogryzko, V., Fuller-Pace, F. V., Hoffman, E. P., Tapscott, S. J., Sartorelli, V. The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. Dev. Cell 11: 547-560, 2006.<br /><br />3. Iggo, R., Gough, A., Xu, W., Lane, D. P., Spurr, N. K. Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc. Nat. Acad. Sci. 86: 6211-6214, 1989.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21007199-1	PA1964	-	100 ug	$370		Add to Cart
BHA21007199-2	PA1964-10ug	Sample	10 ug	$99		Add to Cart
BHA21007199-3	PA1964-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21007199-4	PA1964-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21007199-5	PA1964-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21007199-6	PA1964-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21007199-7	PA1964-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21007199-8	PA1964-FITC	FITC	100 ug	$570		Add to Cart
BHA21007199-9	PA1964-HRP	HRP	100 ug	$570		Add to Cart
BHA21007199-10	PA1964-APC	APC	100 ug	$670		Add to Cart
BHA21007199-11	PA1964-PE	PE	100 ug	$670		Add to Cart
BHA21007199-12	PA1964-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21007199-13	PA1964-carrier-free	carrier-free	100 ug	$370		Add to Cart

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