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Home / Primary Antibodies / Polyclonal Antibodies

Anti-DCTN1/p150-glued Antibody Picoband®

SKU : BHA21003283

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Mfr.No.	A02175-2, A02175-2-10ug, A02175-2-Biotin, A02175-2-Cy3, A02175-2-Dylight488, A02175-2-Dylight550, A02175-2-Dylight594, A02175-2-FITC, A02175-2-HRP, A02175-2-APC, A02175-2-PE, A02175-2-iFluor647, A02175-2-carrier-free
Alternative Names	betacellulin
Background	Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene. This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains ly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alteative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-DCTN1/p150-glued Antibody Picoband® catalog # A02175-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Host	Rabbit
Immunogen Region	E.coli-derived human DCTN1/p150-glued recombinant protein (Position: K1042-S1278).
Isotype	Rabbit IgG
Molecular Weight	150 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Probetacellulin; Betacellulin; BTC; BTC
Tissue Specificity	Highly expressed in testis.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Uniprot	Q14203
Gene Id	1639
Gene Name	DCTN1
Subcellular Location	Betacellulin: Secreted, extracellular space.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Caroppo, P., Le Ber, I., Clot, F., Rivaud-Pechoux, S., Camuzat, A., De Septenville, A., Boutoleau-Bretonniere, C., Mourlon, V., Sauvee, M., Lebouvier, T., Bonnet, A.-M., Levy, R., Vercelletto, M., Brice, A. DCTN1 mutation analysis in families with progressive supranuclear palsy-like phenotypes. JAMA Neurol. 71: 208-215, 2014. <br /><br />2. Collin, G. B., Nishina, P. M., Marshall, J. D., Naggert, J. K. Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome. Genomics 53: 359-364, 1998. <br /><br />3. Eaton, B. A., Fetter, R. D., Davis, G. W. Dynactin is necessary for synapse stabilization. Neuron 34: 729-741, 2002. <br /><br /><br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003283-1	A02175-2	-	100 ug	$370		Add to Cart
BHA21003283-2	A02175-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21003283-3	A02175-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003283-4	A02175-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003283-5	A02175-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003283-6	A02175-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003283-7	A02175-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003283-8	A02175-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21003283-9	A02175-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21003283-10	A02175-2-APC	APC	100 ug	$670		Add to Cart
BHA21003283-11	A02175-2-PE	PE	100 ug	$670		Add to Cart
BHA21003283-12	A02175-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003283-13	A02175-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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