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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Collagen III/COL3A1 Antibody Picoband®

SKU : BHA21002051

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Mfr.No.	A00788-3, A00788-3-10ug, A00788-3-Biotin, A00788-3-Cy3, A00788-3-Dylight488, A00788-3-Dylight550, A00788-3-Dylight594, A00788-3-FITC, A00788-3-HRP, A00788-3-APC, A00788-3-PE, A00788-3-iFluor647, A00788-3-carrier-free
Alternative Names	collagen, type III, alpha 1
Background	COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of inteal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Clonality	Polyclonal
Cross Reactivity	No cross reactivity with other proteins.
Description	Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband® catalog # A00788-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the
Host	Rabbit
Immunogen Region	E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455).
Isotype	Rabbit IgG
Molecular Weight	200 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Collagen alpha-1 (III) chain; COL3A1
Tissue Specificity	Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	P02461
Gene Id	1281
Gene Name	COL3A1
Subcellular Location	Extracellular matrix.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, IHC, WB, Flow Cytometry
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml<br> Flow Cytometry(Fixed), 1-3 ug/1x10<sup>6</sup> cells<br> ELISA, 0.1-0.5ug/ml<br>

Reference	1. Janeczko RA, Ramirez F (Oct 1989). "Nucleotide and amino acid sequences of the entire human alpha 1 (III) collagen". Nucleic Acids Res 17 (16): 6742.<br /><br />2. Superti-Furga A, Gugler E, Gitzelmann R, Steinmann B (Jun 1988). "Ehlers-Danlos syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen". J Biol Chem 263 (13): 622632.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002051-1	A00788-3	-	100 ug	$370		Add to Cart
BHA21002051-2	A00788-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21002051-3	A00788-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002051-4	A00788-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002051-5	A00788-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002051-6	A00788-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002051-7	A00788-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002051-8	A00788-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21002051-9	A00788-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21002051-10	A00788-3-APC	APC	100 ug	$670		Add to Cart
BHA21002051-11	A00788-3-PE	PE	100 ug	$670		Add to Cart
BHA21002051-12	A00788-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002051-13	A00788-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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