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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Claudin 3/CLDN3 Antibody Picoband®

SKU : BHA21003279

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Mfr.No.	A04393-5, A04393-5-10ug, A04393-5-Biotin, A04393-5-Cy3, A04393-5-Dylight488, A04393-5-Dylight550, A04393-5-Dylight594, A04393-5-FITC, A04393-5-HRP, A04393-5-APC, A04393-5-PE, A04393-5-iFluor647, A04393-5-carrier-free
Alternative Names	acyl-CoA synthetase long chain family member 4
Background	Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Claudin 3/CLDN3 Antibody Picoband® catalog # A04393-5. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially activates arachidonate and eicos
Host	Rabbit
Immunogen Region	E.coli-derived human Claudin 3/CLDN3 recombinant protein (Position: A101-V220).
Isotype	Rabbit IgG
Molecular Weight	23 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	"Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACSL4; ACS4, FACL4, LACS4 "
Tissue Specificity	Expressed in the prostate gland and prostate cancers.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Uniprot	O15551
Gene Id	1365
Gene Name	CLDN3
Subcellular Location	Secreted.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.1-0.25ug/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003.<br /><br />2. Dube, E., Chan, P. T. K., Hermo, L., Cyr, D. G. Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis. Biol. Reprod. 76: 1034-1044, 2007.<br /><br />3. Furuse, M., Fujita, K., Hiiragi, T., Fujimoto, K., Tsukita, S. Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J. Cell Biol. 141: 1539-1550, 1998

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003279-1	A04393-5	-	100 ug	$370		Add to Cart
BHA21003279-2	A04393-5-10ug	Sample	10 ug	$99		Add to Cart
BHA21003279-3	A04393-5-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003279-4	A04393-5-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003279-5	A04393-5-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003279-6	A04393-5-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003279-7	A04393-5-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003279-8	A04393-5-FITC	FITC	100 ug	$570		Add to Cart
BHA21003279-9	A04393-5-HRP	HRP	100 ug	$570		Add to Cart
BHA21003279-10	A04393-5-APC	APC	100 ug	$670		Add to Cart
BHA21003279-11	A04393-5-PE	PE	100 ug	$670		Add to Cart
BHA21003279-12	A04393-5-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003279-13	A04393-5-carrier-free	carrier-free	100 ug	$370		Add to Cart

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