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Home / Primary Antibodies / Polyclonal Antibodies

Anti-CDK1 Antibody Picoband®

SKU : BHA21000429

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Mfr.No.	PB9533, PB9533-10ug, PB9533-Biotin, PB9533-Cy3, PB9533-Dylight488, PB9533-Dylight550, PB9533-Dylight594, PB9533-FITC, PB9533-HRP, PB9533-APC, PB9533-PE, PB9533-iFluor647, PB9533-carrier-free
Alternative Names	Cyclin-dependent kinase 1
Background	CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-CDK1 Antibody Picoband® catalog # PB9533. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in
Host	Rabbit
Immunogen Region	E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.
Isotype	Rabbit IgG
Molecular Weight	34 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Cyclin-dependent kinase 1;CDK1;2.7.11.22;2.7.11.23;Cell division control protein 2 homolog;Cell division protein kinase 1;p34 protein kinase;CDK1;CDC2, CDC28A, CDKN1, P34CDC2;
Tissue Specificity	Isoform 2 is found in breast cancer tissues.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P06493
Gene Id	983
Gene Name	CDK1
Sequence	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Subcellular Location	Nucleus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin- B1. Accumulates in mitochondria in G2-arrested cells upon DNA- damage.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, IHC-F, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Human Immunocytochemistry/Immunofluorescence, 2ug/ml, Human Flow Cytometry (Fixed), 1-3ug/1x10⁶ cells, Human

Reference	1. Draetta, G.; Piwnica-Worms, H.; Morrison, D.; Druker, B.; Roberts, T.; Beach, D.: Human CDC2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336: 738-744, 1988. 2. Nazarenko, S. A.; Ostroverhova, N. V.; Spurr, N. K. : Regional assignment of the human cell cycle control gene CDC2 to chromosome 10q21 by in situ hybridization. Hum. Genet. 87: 621-622, 1991. 3. Spurr, N. K.; Goodfellow, P. N.; Nurse, P.; Lee, M. : Assignment of the human homologue of the yeast cell cycle control gene CDC2 to chromosome 10. (Abstract) Cytogenet. Cell Genet. 46: 698, 1987.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000429-1	PB9533	100 ug	-	$370		Add to Cart
BHA21000429-2	PB9533-10ug	10 ug	Sample	$99		Add to Cart
BHA21000429-3	PB9533-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000429-4	PB9533-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000429-5	PB9533-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000429-6	PB9533-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000429-7	PB9533-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000429-8	PB9533-FITC	100 ug	FITC	$570		Add to Cart
BHA21000429-9	PB9533-HRP	100 ug	HRP	$570		Add to Cart
BHA21000429-10	PB9533-APC	100 ug	APC	$670		Add to Cart
BHA21000429-11	PB9533-PE	100 ug	PE	$670		Add to Cart
BHA21000429-12	PB9533-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000429-13	PB9533-carrier-free	100 ug	carrier-free	$370		Add to Cart

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