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Home / Primary Antibodies / Polyclonal Antibodies

Anti-CDC27 Antibody Picoband®

SKU : BHA21002995

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Mfr.No.	A03905-3, A03905-3-10ug, A03905-3-Biotin, A03905-3-Cy3, A03905-3-Dylight488, A03905-3-Dylight550, A03905-3-Dylight594, A03905-3-FITC, A03905-3-HRP, A03905-3-APC, A03905-3-PE, A03905-3-iFluor647, A03905-3-carrier-free
Alternative Names	aldolase, fructose-bisphosphate B
Background	Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alteative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-CDC27 Antibody Picoband® catalog # A03905-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocate
Host	Rabbit
Immunogen Region	E.coli-derived human CDC27 recombinant protein (Position: H609-F824).
Isotype	Rabbit IgG
Molecular Weight	92 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Fructose-bisphosphate aldolase B; Liver-type aldolase; ALDOB; ALDB
Tissue Specificity	Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Uniprot	P30260
Gene Id	996
Gene Name	CDC27
Subcellular Location	Nucleus. Cytoplasm.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Adams, M. D., Dubnick, M., Kerlavage, A. R., Moreno, R., Kelley, J. M., Utterback, T. R., Nagle, J. W., Fields, C., Venter, J. C. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992.<br /><br />2. Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001.<br /><br />3. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Ko, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002995-1	A03905-3	-	100 ug	$370		Add to Cart
BHA21002995-2	A03905-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21002995-3	A03905-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002995-4	A03905-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002995-5	A03905-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002995-6	A03905-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002995-7	A03905-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002995-8	A03905-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21002995-9	A03905-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21002995-10	A03905-3-APC	APC	100 ug	$670		Add to Cart
BHA21002995-11	A03905-3-PE	PE	100 ug	$670		Add to Cart
BHA21002995-12	A03905-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002995-13	A03905-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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