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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Cd80 Antibody Picoband®

SKU : BHA21004003

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Mfr.No.	A00196-3, A00196-3-10ug, A00196-3-Biotin, A00196-3-Cy3, A00196-3-Dylight488, A00196-3-Dylight550, A00196-3-Dylight594, A00196-3-FITC, A00196-3-HRP, A00196-3-APC, A00196-3-PE, A00196-3-iFluor647, A00196-3-carrier-free
Alternative Names	CD80 molecule
Background	Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Cd80 Antibody Picoband® catalog # A00196-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Host	Rabbit
Immunogen Region	E.coli-derived mouse Cd80 recombinant protein (Position: D62-K92).
Isotype	Rabbit IgG
Molecular Weight	70 kDa
Reactivity	Human, Mouse
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80; CD80; CD28LG; CD28LG1; LAB7
Tissue Specificity	Hematopoietic stem and progenitor cell- enriched populations. Found in brain, placenta and testis.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q00609
Gene Id	12519
Gene Name	Cd80
Subcellular Location	Membrane; Single-pass type I membrane protein.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	ELISA, Flow Cytometry, WB
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse<br> Flow Cytometry (Fixed), 1-3 ug/1x10<sup>6</sup> cells, Mouse<br> ELISA, 0.1-0.5 ug/ml, -<br>

Reference	1. Peach, R J; Bajorath J, Naemura J, Leytze G, Greene J, Aruffo A, Linsley P S (Sep. 1995). "Both extracellular immunoglobin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28". J. Biol. Chem. (UNITED STATES) 270 (36): 211817.<br /><br />2. Stamper, C C; Zhang Y, Tobin J F, Erbe D V, Ikemizu S, Davis S J, Stahl M L, Seehra J, Somers W S, Mosyak L (Mar. 2001). "Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses". Nature (England) 410 (6828): 60811.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21004003-1	A00196-3	-	100 ug	$370		Add to Cart
BHA21004003-2	A00196-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21004003-3	A00196-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21004003-4	A00196-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21004003-5	A00196-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21004003-6	A00196-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21004003-7	A00196-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21004003-8	A00196-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21004003-9	A00196-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21004003-10	A00196-3-APC	APC	100 ug	$670		Add to Cart
BHA21004003-11	A00196-3-PE	PE	100 ug	$670		Add to Cart
BHA21004003-12	A00196-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21004003-13	A00196-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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