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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Caspase 3/CASP3 Antibody Picoband®

SKU : BHA21007111

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Mfr.No.	PA1849, PA1849-10ug, PA1849-Biotin, PA1849-Cy3, PA1849-Dylight488, PA1849-Dylight550, PA1849-Dylight594, PA1849-FITC, PA1849-HRP, PA1849-APC, PA1849-PE, PA1849-iFluor647, PA1849-carrier-free
Alternative Names	Caspase-3
Background	Caspase 3 (caspase 3, apoptosis-related cysteine peptidase) is a caspase protein that interacts with caspase 8 and caspase 9, also known as Caspase-3, PARP CLEAVAGE PROTEASE, APOPAIN, CPP32, CPP32B, YAMA. It is a member of the cysteine-aspartic acid protease (caspase) family. PCR analysis of 16 human tissues revealed expression of full-length CASP3, as well as CASP3s at somewhat lower levels, in all tissues tested. Weste blot analysis of 3 cell lines revealed the prominent CASP3 band at 32 kD and CASP3s at 20 kD. Several human cancer cell lines showed coexpression of both variants at the mRNA and protein levels. Overexpression of the catalytically inactive CASP3s by human kidney cells offered some resistance to inducers of apoptosis, and CASP3s accumulation could be enhanced with addition of proteasome inhibitors. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Alteative splicing of this gene results in two transcript variants that encode the same protein. Encoded by the CASP3 gene, CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Nicholson et al. developed a potent peptide aldehyde inhibitor and showed that it prevented apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Caspase 3/CASP3 Antibody catalog # PA1849. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element bin
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminal of human Caspase 3, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	31 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32;
Tissue Specificity	Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Uniprot	P42574
Gene Name	CASP3
Sequence	Belongs to the peptidase C14A family.
Subcellular Location	Cytoplasm.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Rat, Mouse<br>

Reference	1. Huang, Y., Shin, N.-H., Sun, Y., Wang, K. K. W.Molecular cloning and characterization of a novel caspase-3 variant that attenuates apoptosis induced by proteasome inhibition.Biochem. Biophys. Res. Commun. 283: 762-769, 2001.<br /><br />2. Nicholson, D. W., Ali, A., Thoberry, N. A., Vaillancourt, J. P., Ding, C. K., Gallant, M., Gareau, Y., Griffin, P. R., Labelle, M., Lazebnik, Y. A., Munday, N. A., Raju, S. M., Smulson, M. E., Yamin, T.-T., Yu, V. L., Miller, D. K. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37-43, 1995.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21007111-1	PA1849	-	100 ug	$370		Add to Cart
BHA21007111-2	PA1849-10ug	Sample	10 ug	$99		Add to Cart
BHA21007111-3	PA1849-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21007111-4	PA1849-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21007111-5	PA1849-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21007111-6	PA1849-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21007111-7	PA1849-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21007111-8	PA1849-FITC	FITC	100 ug	$570		Add to Cart
BHA21007111-9	PA1849-HRP	HRP	100 ug	$570		Add to Cart
BHA21007111-10	PA1849-APC	APC	100 ug	$670		Add to Cart
BHA21007111-11	PA1849-PE	PE	100 ug	$670		Add to Cart
BHA21007111-12	PA1849-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21007111-13	PA1849-carrier-free	carrier-free	100 ug	$370		Add to Cart

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