JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Primary Antibodies / Polyclonal Antibodies

Anti-Bub3 Antibody Picoband®

SKU : BHA21006953

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Boster

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	PA1645, PA1645-10ug, PA1645-Biotin, PA1645-Cy3, PA1645-Dylight488, PA1645-Dylight550, PA1645-Dylight594, PA1645-FITC, PA1645-HRP, PA1645-APC, PA1645-PE, PA1645-iFluor647, PA1645-carrier-free
Alternative Names	Mitotic checkpoint protein BUB3
Background	BUB3 (BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC); though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Bub3 Antibody catalog # PA1645. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of B
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence in the middle region of human Bub3, identical to the related rat and mouse sequences.
Isotype	Rabbit IgG
Molecular Weight	37 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Mitotic checkpoint protein BUB3;BUB3;
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	O43684
Gene Name	BUB3
Sequence	Belongs to the WD repeat BUB3 family.
Subcellular Location	Nucleus. Chromosome, centromere, kinetochore . Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, IHC-F, ICC, WB
Description	Immunocytochemistry , 0.5-1ug/ml, Human, Mouse, Rat<br>Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Rat, Human, Mouse<br>Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat<br>Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br>

Reference	1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C. Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999.<br /><br />2. Kalitsis, P., Earle, E., Fowler, K. J., Choo, K. H. A. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 14: 2277-2282, 2000.<br /><br />3. Morgan, David O (2007). The cell cycle: principles of control. London: Published by New Science Press in association with Oxford University Press.<br /><br />

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006953-1	PA1645	-	100 ug	$370		Add to Cart
BHA21006953-2	PA1645-10ug	Sample	10 ug	$99		Add to Cart
BHA21006953-3	PA1645-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006953-4	PA1645-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006953-5	PA1645-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006953-6	PA1645-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006953-7	PA1645-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006953-8	PA1645-FITC	FITC	100 ug	$570		Add to Cart
BHA21006953-9	PA1645-HRP	HRP	100 ug	$570		Add to Cart
BHA21006953-10	PA1645-APC	APC	100 ug	$670		Add to Cart
BHA21006953-11	PA1645-PE	PE	100 ug	$670		Add to Cart
BHA21006953-12	PA1645-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006953-13	PA1645-carrier-free	carrier-free	100 ug	$370		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.