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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Bid Antibody Picoband®

SKU : BHA21001221

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Mfr.No.	A00730, A00730-10ug, A00730-Biotin, A00730-Cy3, A00730-Dylight488, A00730-Dylight550, A00730-Dylight594, A00730-FITC, A00730-HRP, A00730-APC, A00730-PE, A00730-iFluor647, A00730-carrier-free
Alternative Names	BH3-interacting domain death agonist
Background	BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, the human BID gene is mapped to 22q11. It is reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Bid Antibody Picoband® catalog # A00730. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c. .
Host	Rabbit
Immunogen Region	E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively.
Isotype	Rabbit IgG
Molecular Weight	22 kDa
Reactivity	Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	BH3-interacting domain death agonist;p22 BID;BID;BH3-interacting domain death agonist p15;p15 BID;BH3-interacting domain death agonist p13;p13 BID;BH3-interacting domain death agonist p11;p11 BID;Bid;
Tissue Specificity	Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	P70444
Gene Id	12122
Gene Name	Bid
Subcellular Location	Cytoplasm . Mitochondrion membrane . When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IHC, WB
Description	Weste blot, 0.1-0.5ug/ml, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Rat, By Heat<br> Flow Cytometry (Fixed), 1-3 ug/1x10<sup>6</sup> cells, Mouse<br>

Reference	1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. <br /><br />2. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998.<br /><br />3. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. <br /><br />

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001221-1	A00730	100 ug	-	$370		Add to Cart
BHA21001221-2	A00730-10ug	10 ug	Sample	$99		Add to Cart
BHA21001221-3	A00730-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001221-4	A00730-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001221-5	A00730-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001221-6	A00730-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001221-7	A00730-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001221-8	A00730-FITC	100 ug	FITC	$570		Add to Cart
BHA21001221-9	A00730-HRP	100 ug	HRP	$570		Add to Cart
BHA21001221-10	A00730-APC	100 ug	APC	$670		Add to Cart
BHA21001221-11	A00730-PE	100 ug	PE	$670		Add to Cart
BHA21001221-12	A00730-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001221-13	A00730-carrier-free	100 ug	carrier-free	$370		Add to Cart

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