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Home / Primary Antibodies / Polyclonal Antibodies

Anti-BCL9L Antibody Picoband®

SKU : BHA21002179

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Mfr.No.	A05905, A05905-10ug, A05905-Biotin, A05905-Cy3, A05905-Dylight488, A05905-Dylight550, A05905-Dylight594, A05905-FITC, A05905-HRP, A05905-APC, A05905-PE, A05905-iFluor647, A05905-carrier-free
Alternative Names	BCL9 like
Background	B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514-517 and 970-973), and one poly-Ala segment (aa 900-903). There is one potential alteate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 13911426. Over aa 1009-1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-BCL9L Antibody Picoband® catalog # A05905. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1.
Host	Rabbit
Immunogen Region	E.coli-derived human BCL9L recombinant protein (Position: E397-A666).
Isotype	Rabbit IgG
Molecular Weight	200 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	B-cell CLL/lymphoma 9-like protein; B-cell lymphoma 9-like protein; BCL9-like protein; Protein BCL9-2; BCL9L; DLNB11
Tissue Specificity	Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q86UU0
Gene Id	283149
Gene Name	BCL9L
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Brembeck, F. H., Schwarz-Romond, T., Bakkers, J., Wilhelm, S., Hammerschmidt, M., Birchmeier, W. Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions. Genes Dev. 18: 2225-2230, 2004.<br /><br />2. Katoh, M., Katoh, M. Identification and characterization of human BCL9L gene and mouse Bcl9l gene in silico. Int. J. Molec. Med. 12: 643-649, 2003.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002179-1	A05905	-	100 ug	$370		Add to Cart
BHA21002179-2	A05905-10ug	Sample	10 ug	$99		Add to Cart
BHA21002179-3	A05905-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002179-4	A05905-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002179-5	A05905-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002179-6	A05905-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002179-7	A05905-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002179-8	A05905-FITC	FITC	100 ug	$570		Add to Cart
BHA21002179-9	A05905-HRP	HRP	100 ug	$570		Add to Cart
BHA21002179-10	A05905-APC	APC	100 ug	$670		Add to Cart
BHA21002179-11	A05905-PE	PE	100 ug	$670		Add to Cart
BHA21002179-12	A05905-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002179-13	A05905-carrier-free	carrier-free	100 ug	$370		Add to Cart

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