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Home / Primary Antibodies / Polyclonal Antibodies

Anti-ATG7 Antibody Picoband®

SKU : BHA21002902

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Mfr.No.	A00346-4, A00346-4-10ug, A00346-4-Biotin, A00346-4-Cy3, A00346-4-Dylight488, A00346-4-Dylight550, A00346-4-Dylight594, A00346-4-FITC, A00346-4-HRP, A00346-4-APC, A00346-4-PE, A00346-4-iFluor647, A00346-4-carrier-free
Alternative Names	autophagy related 7
Background	Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alteative splicing results in multiple transcript variants.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-ATG7 Antibody Picoband® catalog # A00346-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylet
Host	Rabbit
Immunogen Region	E.coli-derived human ATG7 recombinant protein (Position: D7-Q690).
Isotype	Rabbit IgG
Molecular Weight	78 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; ATG7; APG7L
Tissue Specificity	Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Uniprot	O95352
Gene Id	10533
Gene Name	ATG7
Subcellular Location	Cytoplasm. Preautophagosomal structure.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, WB
Description	Weste blot, 0.25-0.5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Komatsu, M., Wang, Q. J., Holstein, G. R., Friedrich, V. L., Jr., Iwata, J., Kominami, E., Chait, B. T., Tanaka, K., Yue, Z. Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc. Nat. Acad. Sci. 104: 14489-14494, 2007.<br /><br />2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Erratum: Science 341: 457 only, 2013.<br /><br />3. Nishida, Y., Arakawa, S., Fujitani, K., Yamaguchi, H., Mizuta, T., Kanaseki, T., Komatsu, M., Otsu, K., Tsujimoto, Y., Shimizu, S. Discovery of Atg5/Atg7-independent alteative macroautophagy. Nature 461: 654-658, 2009. Note: Erratum: Nature 533: 130 only, 2016.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002902-1	A00346-4	-	100 ug	$370		Add to Cart
BHA21002902-2	A00346-4-10ug	Sample	10 ug	$99		Add to Cart
BHA21002902-3	A00346-4-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002902-4	A00346-4-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002902-5	A00346-4-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002902-6	A00346-4-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002902-7	A00346-4-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002902-8	A00346-4-FITC	FITC	100 ug	$570		Add to Cart
BHA21002902-9	A00346-4-HRP	HRP	100 ug	$570		Add to Cart
BHA21002902-10	A00346-4-APC	APC	100 ug	$670		Add to Cart
BHA21002902-11	A00346-4-PE	PE	100 ug	$670		Add to Cart
BHA21002902-12	A00346-4-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002902-13	A00346-4-carrier-free	carrier-free	100 ug	$370		Add to Cart

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