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Home / Primary Antibodies / Polyclonal Antibodies

Anti-AMFR Antibody Picoband®

SKU : BHA21000944

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Mfr.No.	PB10039, PB10039-10ug, PB10039-Biotin, PB10039-Cy3, PB10039-Dylight488, PB10039-Dylight550, PB10039-Dylight594, PB10039-FITC, PB10039-HRP, PB10039-APC, PB10039-PE, PB10039-iFluor647, PB10039-carrier-free
Alternative Names	E3 ubiquitin-protein ligase AMFR
Background	Autocrine motility factor receptor, isoform 2 is a protein that in humans is encoded by the AMFR gene. Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-AMFR Antibody Picoband® catalog # PB10039. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulu
Host	Rabbit
Immunogen Region	E. coli-derived human AMFR recombinant protein (Position: E553-S643). Human AMFR shares 89% amino acid (aa) sequence identity with mouse AMFR.
Isotype	Rabbit IgG
Molecular Weight	73 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	E3 ubiquitin-protein ligase AMFR;6.3.2.-;Autocrine motility factor receptor;AMF receptor;RING finger protein 45;gp78;AMFR;RNF45;
Tissue Specificity	Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in adult peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q9UKV5
Gene Id	267
Gene Name	AMFR
Subcellular Location	Endoplasmic reticulum membrane ; Multi-pass membrane protein .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human Flow Cytometry (Fixed), 1-3ug/1x10⁶ cells, Human

Reference	1. Lee, Joon No; Zhang Xiangyu; Feramisco Jamison D; Gong Yi; Ye Jin (Nov 2008). "Unsaturated fatty acids inhibit proteasomal degradation of Insig-1 at a postubiquitination step". J. Biol. Chem. (United States)283 (48): 3377283. 2. Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A (Aug 1991). "Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor". J Biol Chem 266 (20): 134428.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000944-1	PB10039	100 ug	-	$370		Add to Cart
BHA21000944-2	PB10039-10ug	10 ug	Sample	$99		Add to Cart
BHA21000944-3	PB10039-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000944-4	PB10039-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000944-5	PB10039-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000944-6	PB10039-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000944-7	PB10039-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000944-8	PB10039-FITC	100 ug	FITC	$570		Add to Cart
BHA21000944-9	PB10039-HRP	100 ug	HRP	$570		Add to Cart
BHA21000944-10	PB10039-APC	100 ug	APC	$670		Add to Cart
BHA21000944-11	PB10039-PE	100 ug	PE	$670		Add to Cart
BHA21000944-12	PB10039-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000944-13	PB10039-carrier-free	100 ug	carrier-free	$370		Add to Cart

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