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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Alpha A Crystallin/CRYAA Antibody Picoband®

SKU : BHA21000841

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Mfr.No.	PB9940, PB9940-10ug, PB9940-Biotin, PB9940-Cy3, PB9940-Dylight488, PB9940-Dylight550, PB9940-Dylight594, PB9940-FITC, PB9940-HRP, PB9940-APC, PB9940-PE, PB9940-iFluor647, PB9940-carrier-free
Alternative Names	Alpha-crystallin A chain
Background	Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Alpha A Crystallin/CRYAA Antibody Picoband® catalog # PB9940. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. .
Host	Rabbit
Immunogen Region	E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.
Isotype	Rabbit IgG
Molecular Weight	20 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Alpha-crystallin A chain;Heat shock protein beta-4;HspB4;Alpha-crystallin A (1-172);Alpha-crystallin A (1-168);Alpha-crystallin A (1-162);CRYAA;CRYA1, HSPB4;
Tissue Specificity	Expressed in eye lens. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P02489
Gene Id	1409
Gene Name	CRYAA
Subcellular Location	Cytoplasm . Nucleus . Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IHC, IHC-F, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Mouse, Rat, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Human Immunocytochemistry, 0.5-1ug/ml, Human Flow Cytometry (Fixed), 1-3ug/1x10⁶ cells, Human

Reference	1. "Entrez Gene: CRYAA crystallin, alpha A". 2. Derham BK, Harding JJ (1999). "Alpha-crystallin as a molecular chaperone.". Progress in retinal and eye research 18 (4): 463509. 3. Jaworski CJ, Piatigorsky J (1989). "A pseudo-exon in the functional human alpha A-crystallin gene.". Nature337 (6209): 7524.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000841-1	PB9940	100 ug	-	$370		Add to Cart
BHA21000841-2	PB9940-10ug	10 ug	Sample	$99		Add to Cart
BHA21000841-3	PB9940-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000841-4	PB9940-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000841-5	PB9940-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000841-6	PB9940-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000841-7	PB9940-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000841-8	PB9940-FITC	100 ug	FITC	$570		Add to Cart
BHA21000841-9	PB9940-HRP	100 ug	HRP	$570		Add to Cart
BHA21000841-10	PB9940-APC	100 ug	APC	$670		Add to Cart
BHA21000841-11	PB9940-PE	100 ug	PE	$670		Add to Cart
BHA21000841-12	PB9940-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000841-13	PB9940-carrier-free	100 ug	carrier-free	$370		Add to Cart

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