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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Agrin/AGRN Picoband® Antibody

SKU : BHA21002511

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Mfr.No.	A04649, A04649-10ug, A04649-Biotin, A04649-Cy3, A04649-Dylight488, A04649-Dylight550, A04649-Dylight594, A04649-FITC, A04649-HRP, A04649-APC, A04649-PE, A04649-iFluor647, A04649-carrier-free
Alternative Names	agrin
Background	Agrin is a protein that in humans is encoded by the AGRN gene. It is mapped to 1p36.33. This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alteative splicing results in multiple transcript variants encoding different isoforms.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Agrin/AGRN Picoband® Antibody catalog # A04649. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and s key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the pho
Host	Rabbit
Immunogen Region	E.coli-derived human Agrin/AGRN recombinant protein (Position: S1864-P2068).
Isotype	Rabbit IgG
Molecular Weight	205-217 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Agrin; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; AGRN; AGRIN
Tissue Specificity	Brain specific. Detected in neuronal cells.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	O00468
Gene Id	375790
Gene Name	AGRN
Subcellular Location	Extracellular matrix. Cell membrane. Single-pass type II membrane protein. Synapse.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Bassat, E., Mutlak, Y. E., Genzelinakh, A., Shadrin, I. Y., Umansky, K. B., Yifa, O., Kain, D., Rajchman, D., Leach, J., Bassat, D. R., Udi, Y., Sarig, R., Sagi, I., Martin, J. F., Bursac, N., Cohen, S., Tzahor, E. The extracellular matrix protein agrin promotes heart regeneration in mice. Nature 547: 179-184, 2017.<br /><br />2. Burgess, R. W., Nguyen, Q.T., Son, Y. J., Lichtman, J. W., Sanes, J. R. Alteatively spliced isoforms of nerve- and muscle-derived agrin: their roles at the neuromuscular junction. Neuron 23: 33-44, 1999.<br /><br />3. Campanelli, J. T., Hoch, W., Rupp, F., Kreiner, T., Scheller, R. H. Agrin mediates cell contact-induced acetylcholine receptor clustering. Cell 67: 909-916, 1991.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002511-1	A04649	-	100 ug	$370		Add to Cart
BHA21002511-2	A04649-10ug	Sample	10 ug	$99		Add to Cart
BHA21002511-3	A04649-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002511-4	A04649-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002511-5	A04649-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002511-6	A04649-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002511-7	A04649-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002511-8	A04649-FITC	FITC	100 ug	$570		Add to Cart
BHA21002511-9	A04649-HRP	HRP	100 ug	$570		Add to Cart
BHA21002511-10	A04649-APC	APC	100 ug	$670		Add to Cart
BHA21002511-11	A04649-PE	PE	100 ug	$670		Add to Cart
BHA21002511-12	A04649-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002511-13	A04649-carrier-free	carrier-free	100 ug	$370		Add to Cart

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