Alkaline phosphatase removes phosphate groups from the 5-prime end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non-tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the N-terminus of rat ALPL (FVPEKEKDPSYWRQQ) was used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.
Isotype
IgG
Predicted Reactivity
Mouse, Rat
Reactivity
Rat, Mouse
Recombinant
No
Antigen
Alkaline Phosphatase
Uniprot
P08289
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the Alkaline Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.