Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, known as tissue-nonspecific, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase. Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids 21-35 (EKEKDPKYWRDQAQE) of human ALPL were used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Antigen
Alkaline Phosphatase
Uniprot
P05186
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
The lyophilized Alkaline Phosphatase antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml