ADP-ribosylation factor 6 (ARF6) is a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids R75-S175) was used as the immunogen for the ADP-ribosylation factor 6 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Mouse, Rat, Human
Recombinant
No
Subcellular Location
Cytoplasmic, cell membrane
Antigen
ADP-ribosylation factor 6
Uniprot
P62330
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Format
Purified
Purification
Antigen affinity purified
Storage
After reconstitution, the ADP-ribosylation factor 6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
FACS, IF, WB, Direct ELISA
Dilution
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml