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Home / ELISA Kit / PharmaTest ELISA Kits

GCSF ADA (granulocyte colony stimulating factor) ELISA

SKU : BHE18300020

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Mfr.No.	EL-141-73196
Detection Range	45 ng/mL -1440 ng/mL
Reactivity	Human
Assay Duration	2.5 hours
Assay Type	Direct, Sandwich
Description	Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream Reagent preparation Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation:Dilute wash buffer concentrate with ultra-pure water 1/20 before use (for example, add 50mL wash buffer concentrate to 450mL ultra-pure water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11ul concentrate to 11ml of assay buffer). Mix well. 3. Calibrator Preparation: Dilute the calibrator from 1.15mg/ml down to 11.5ug/ml by pipetting 5ul of calibrator stock into 495ul assay buffer. Label "Cal. Int." Mix well. Prepare calibrators with concentrations ranging from 1440ng/ml to 45ng/ml. The following is an example calibrator curve Results calculation 1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. 2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample. Sample preparation Dilute calibrators and test samples 1/50 with assay buffer (for example add 5ul of prepared calibrator or sample to 245ul of assay buffer). Mix well. Do not store diluted samples.
Detection Method	Peroxidase / OD450
Kit Components	Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 ?l 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 ?l TMB 12 ml TMB stop solution
Limit Of Detection	45 ng/mL
Precision	Intra-assay coefficient variation (CV) <20%, inter-assay CV <20%
Notes	This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
Usage Notes	Quantification of antibodies to G-CSF
Protocol	This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.

Applications	ELISA

Storage	-20C, 1 year

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SKU	Supplier No.	Size	Your price	Quantity
BHE18300020	EL-141-73196	1X96 wells	$809.99		Add to Cart

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