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Home / ELISA Kit / PharmaTest ELISA Kits

Anti-PEG antibody ELISA (mouse IgM specific)

SKU : BHE18300016

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Mfr.No.	EL-141-PEG-mIGM
Reactivity	Mouse
Assay Duration	2.5 hours
Assay Type	Direct, Sandwich
Description	Polyethylene glycol (PEG) chains are often used to modify therapeutic biologic agents in order to prolong the circulating half-life of the modified protein. It has been reported that repeat injections of PEGylated proteins can induce anti-PEG antibodies. Reagent preparation Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation Dilute wash buffer concentrate with deionized water 1/10 before use (for example add 20mL concentrate to 180mL deionized water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11ul concentrate to 11ml of assay buffer). Mix well. The following is an example calibrator curve. Results calculation 1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor. Sample preparation Dilute test samples 1/5 with assay buffer before use (for example add 50ul of test sample to 200ul assay buffer). Mix well.
Detection Method	Peroxidase / OD450
Kit Components	Coated microtiter plate, 96 wells QC samples - 6x250ul 10X wash buffer - 50 ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Components	Coated microtiter plate, 96 wells QC samples - 6x250ul 10X wash buffer - 50 ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Precision	Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
Notes	This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
Usage Notes	Quanitification of mouse IgM specific
Protocol	This assay employs the sandwich enzyme immunoassay technique. Immobilized PEG is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Anti-PEG antibodies present in biological matrices is bound by the immobilized PEG. After washing away any unbound substances, enzyme linked anti IgG or IgM antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of anti-PEG antibodies present in test samples. The color development is stopped and the intensity of the color is measured

Applications	ELISA

Storage	-20C, 1 year

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SKU	Supplier No.	Size	Your price	Quantity
BHE18300016	EL-141-PEG-mIGM	1X96 wells	$809.99		Add to Cart

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