Syntaxin 1 Blocking Peptide (#BLP-NR002) is the original antigen used for immunization during Anti-Syntaxin 1 Antibody (#ANR-002) generation. The blocking peptide binds and 'blocks' Anti-Syntaxin 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Reconstitution
100 ul PBS
Formulation
Lyophilized Powder.
Accession Number
BLP
Concentration
0.6 mg/ml.
Form
Lyophilized powder
Notes
Peptide confirmation: Confirmed by DNA sequence and SDS-PAGE Is Toxin: No Standard quality control of each lot: Western blot analysis. Antibodies part of immunogen: GST fusion protein with the sequence MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQH STLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKK, corresponding to amino acid residues 1-265 of rat syntaxin 1A Antigen preadsorption control: 3 ug fusion protein per 1 ug antibody
UNSPSC Code
41105329
Lead Time
1-2 Business Days
Gene Id
116470
Sequence
GST fusion protein with the sequence MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQH STLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKK, corresponding to amino acid residues 1-265 of rat syntaxin 1A
Target
STX1A
Country Of Origin
Israel/IL
Shipping
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Purity
>95% (SDS-PAGE)
Storage
Storage After Reconstitution: -20°C. Storage Before Reconstitution: Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C