SNAP-25 Blocking Peptide (#BLP-NR001) is the original antigen used for immunization during Anti-SNAP-25 Antibody (#ANR-001) generation. The blocking peptide binds and 'blocks' Anti-SNAP-25 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Reconstitution
100 ul PBS
Formulation
Lyophilized Powder.
Accession Number
BLP
Concentration
0.5 mg/ml.
Form
Lyophilized powder
Notes
Peptide confirmation: Confirmed by DNA sequence and SDS-PAGE Is Toxin: No Standard quality control of each lot: Western blot analysis. Antibodies part of immunogen: GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25 Antigen preadsorption control: 3 ug fusion protein per 1 ug antibody
UNSPSC Code
41105329
Lead Time
1-2 Business Days
Gene Id
25012
Sequence
GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25
Target
SNAP25
Country Of Origin
Israel/IL
Shipping
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Purity
>95% (SDS-PAGE)
Storage
Storage After Reconstitution: -20°C. Storage Before Reconstitution: Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C