Ultra-Pure BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s Ultra-Pure BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. This strategically-engineered, next generation Taq Polymerase provides rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaqTM has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaqTM can be used with TA cloning vectors. The enzyme is subject to a rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA. Quality control involves a PCR DNA Contamination Test with three different primer sets against host DNA.
Application
Ultra-pure PCR
Super-fast PCR
Storage Condition
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.
Note
Specialized buffer for higher yields, sensitivity, and specificity compared to wild-type Taq polymerase.
Decrease reaction times by 70% using specialized protocol.
For optimal efficiency, use a 25 μl reaction volume.
We recommend assembling no template controls in a DNA-free environment to prevent contamination. Nuclease-free pipette tips and PCR tubes will also improve the results.