Safe-Green™ Opti-DNA Markers contain Safe-Green™ DNA stain which is a non-toxic and efficient alternative DNA visualizing agent to the carcinogenic ethidium bromide (EB). The brightness of Safe-Green™ Opti-DNA Markers is optimized for compatible and proper camera exposure in applications along with PCR products generated from abm’s Safe-Green™ 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use Safe-Green™ Opti-DNA Markers, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source. Safe-Green™ has an excitation maximum at 495 nm and an emission maximum at 520 nm.PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 17 bands, ranging from 50 pb – 1.5 kb, for molecular weight standards in agarose gel electrophoresis. The 200 and 500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
Suggested-load
Resolves into 17 sharp, tight bands.Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Bands
17 bands
Quantity
500 µl/100 loads
Range
50bp-1.5kb
Reaction
Marker
Storage Condition
-20C
Shipping Condition
Ice Packs
Reviews of Safe-Green™ 100bp Opti-DNA Marker